Question

1. What steps do you take to limit growth of microbes in your home? Explain thoroughly.
2. Explain thoroughly how antimicrobial drug resistance develops and explain some steps that can be taken to prevent it from happening.
3. Explain what is meant by a BSL-4 lab. Would you work in one? Why or why not?
4. You are infected with a bacterial disease. Your sister had this same illness last week, and took a full cycle of antibiotics. She quickly became better. You started taking the same antibiotic, but they had no effect. In fact, you had to return to the doctor after a week, because you did not feel better. Why did you remain sick after taking antibiotics, while your sister quickly recovered? Which is the most reasonable hypothesis?

Select one:

a. The bacteria infecting you developed a tolerance for the antibiotic (i.e. individual bacteria experienced a non-genetic change that made them less sensitive to the effects of the antibiotic).

b. You developed a tolerance for the antibiotic (i.e. you experienced a non-genetic change that made you less sensitive to the effects of the antibiotic).

c. The bacteria infecting you evolved to be resistant to the antibiotic (i.e. a genetic mutation for resistance occurred in a bacterial cell, it had a reproductive advantage and increased in the population).

5.-Suppose that scientists want to store a specific bacteria for several years. They believe that they will need to "revive" the bacteria and grow cultures many years down the road as part of their research. Describe the best way to preserve this bacteria.

6.-Some foods are irradiated as a method of eliminating harmful pathogens prior to consumer purchase. What does this mean and is it harmful to the consumer? Explain thoroughly.

7.-Explain thoroughly the difference between a sterilized tool and an aseptic tool.

Microbiology

Answer 1

Q5. Ans The best way to preserve bacteria is to make glycerol stocks. This can be done by adding 500 microlitre of overnight culture with 500 microlitre of 50% glycerol in a screw top tube or cryovial followed by gentle mixing.