EDTA is a metal chelator that chelates to magnesium ions Mg2+ Why is it a problem...
EDTA is a metal chelator that chelates to magnesium ions Mg2+
Why is it a problem to have EDTA during DNA sequencing techniques?
Please explain in detail and any chemistry involved, thanks
Homework Answers
EDTA is a MG2+ Chelator, and Mg2+ is extremely crucial in stabilizing the Polynucleotide Chain by two interactions.
1. It is a cofactor of the DNA Polymerase Enzyme
2. It helps to stabilize the Polynucleotide Chain as is shown below. The phosphates are negatively charged and need to be neutralized during sequencing.
Hence EDTA is a problem for sequencing. In fact, a 1:1 mixture of the two inhibits all activity of the Polymerase! The reaction is : Mg2+ + EDTA4- → MgEDTA2-
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EDTA is a metal chelator that chelates to magnesium ions
Mg2+
Why is it a problem...
EDTA (ethylenediaminetetraacetic acid) is the most widely used metal chelator in analytical chemistry, which forms strong...
EDTA (ethylenediaminetetraacetic acid) is the most widely used metal chelator in analytical chemistry, which forms strong 1:1 complexes with most metal ions. Water hardness can be determined by titrating Ca2+, Mg2+ and other metal ions with EDTA. During the titration, ammonium-ammonium chloride buffer is often used. Which of the following descriptions about the function of ammonium-ammonium chloride is/are correct? To act as a masking agent To form complex with Ca2+ and Mg2+ To be used as a metal ion indicator...
10. EDTA (C10H16N208) is an aminopolycarboxylic acid, widely used for scavenging metal ions. EDTA has high...
10. EDTA (C10H16N208) is an aminopolycarboxylic acid, widely used for scavenging metal ions. EDTA has high denticity, and can displace the H2O ligands that bind to hydrated metal cations. For example: [Fe(H2O).]3+ (aq) + EDTA (aq) → Fe-EDTA'(aq) + 6H20 (1) Explain conceptually, what you think drives the aforementioned reaction (enthalpy or entropy), and why.
i am insure what are the correct answers for this one EDTA Titration 1. EDTA (cthylenediaminetetraacetic...
i am insure what are the correct answers for this one
EDTA Titration 1. EDTA (cthylenediaminetetraacetic acid) is the most widely used metal chelator in analytical chemistry, which forms strong 1:1 complexes with most metal ions. Water hardness can be determined by titrating Ca, Mg and other metal ions with EDTA. During the titration, ammonium-ammonia chloride buffer is often used. Which of the following descriptions about the function of ammonium-ammonia chloride is/are correct? (a) To react with EDTA To form...
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Hardness in groundwater is due to the presence of metal ions, primarily Mg2 and Ca2t. Hardness...
Hardness in groundwater is due to the presence of metal ions, primarily Mg2 and Ca2t. Hardness is generally reported as ppm CaCO3. To measure water hardness, a sample of groundwater is titrated with EDTA, a chelating agent, in the presence of the indicator eriochrome black T, symbolized here as In. Eriochrome black T, a weaker chelating agent than EDTA, is red in the presence of Ca2 and turns blue when Ca2+ is removed. red blue Ca(ln)2+-EDTA → Ca(EDTA,-+ + In...
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Hardness in groundwater is due to the presence of metal ions, primarily Mg2+ and Ca2+. Hardness...
Hardness in groundwater is due to the presence of metal ions, primarily Mg2+ and Ca2+. Hardness is generally reported as ppm CaCO3. To measure water hardness, a sample of groundwater is titrated with EDTA, a chelating agent, in the presence of the indicator Eriochrome Black T, symbolized as In. Eriochrome Black T, a weaker chelating agent than EDTA, is red in the presence of Ca2+ and turns blue when Ca2+ is removed. red blue Ca(In)2+ +EDTACa(EDTA) 2+ In A 50.00...
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enodic Table The magnesium and calcium ions present in seawater ([Mg2+] = 0.059 M and (Ca2+] = 0.011 M) can be separated by selective precipitation with КОН. You may want to reference (Pages 775 - 778) Section 17.6 while completing this problem. Part A If the concentration of Mg2+ in the solution were 0.018 M, what minimum (OH) triggers precipitation of the Mg2+ ion?( Ksp = 2.06 x 10-13.) Express your answer to two significant figures and include the appropriate...
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Please help Why is it necessary to chelate the metal ions from solution during the boiling/lysis...
Please help
Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100C? What would happen if you did not use a chelating agent such as the InstaGene matrix? 1. 2. What is needed from the cells for PCR? 3. What structures must be broken to release DNA from the cell? Why is it necessary to have a primer on each side of the DNA segment to be amplified? 4. 5. How did Ta?...
10. EDTA (C10H16N208) is an aminopolycarboxylic acid, widely used for scavenging metal ions. EDTA has high denticity, and can displace the H2O ligands that bind to hydrated metal cations. For example: [Fe(H2O).]3+ (aq) + EDTA (aq) → Fe-EDTA'(aq) + 6H20 (1) Explain conceptually, what you think drives the aforementioned reaction (enthalpy or entropy), and why.
i am insure what are the correct answers for this one
EDTA Titration 1. EDTA (cthylenediaminetetraacetic acid) is the most widely used metal chelator in analytical chemistry, which forms strong 1:1 complexes with most metal ions. Water hardness can be determined by titrating Ca, Mg and other metal ions with EDTA. During the titration, ammonium-ammonia chloride buffer is often used. Which of the following descriptions about the function of ammonium-ammonia chloride is/are correct? (a) To react with EDTA To form...
Hardness in groundwater is due to the presence of metal ions, primarily Mg2 and Ca2t. Hardness is generally reported as ppm CaCO3. To measure water hardness, a sample of groundwater is titrated with EDTA, a chelating agent, in the presence of the indicator eriochrome black T, symbolized here as In. Eriochrome black T, a weaker chelating agent than EDTA, is red in the presence of Ca2 and turns blue when Ca2+ is removed. red blue Ca(ln)2+-EDTA → Ca(EDTA,-+ + In...
Hardness in groundwater is due to the presence of metal ions, primarily Mg2+ and Ca2+. Hardness is generally reported as ppm CaCO3. To measure water hardness, a sample of groundwater is titrated with EDTA, a chelating agent, in the presence of the indicator Eriochrome Black T, symbolized as In. Eriochrome Black T, a weaker chelating agent than EDTA, is red in the presence of Ca2+ and turns blue when Ca2+ is removed. red blue Ca(In)2+ +EDTACa(EDTA) 2+ In A 50.00...
enodic Table The magnesium and calcium ions present in seawater ([Mg2+] = 0.059 M and (Ca2+] = 0.011 M) can be separated by selective precipitation with КОН. You may want to reference (Pages 775 - 778) Section 17.6 while completing this problem. Part A If the concentration of Mg2+ in the solution were 0.018 M, what minimum (OH) triggers precipitation of the Mg2+ ion?( Ksp = 2.06 x 10-13.) Express your answer to two significant figures and include the appropriate...
Please help
Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100C? What would happen if you did not use a chelating agent such as the InstaGene matrix? 1. 2. What is needed from the cells for PCR? 3. What structures must be broken to release DNA from the cell? Why is it necessary to have a primer on each side of the DNA segment to be amplified? 4. 5. How did Ta?...
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