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4. Based upon your understanding from the lab and lectures, briefly EXPLAIN how/why NIH-3T3 cells are...

4. Based upon your understanding from the lab and lectures, briefly EXPLAIN how/why NIH-3T3 cells are able to adhere to the flask surface (1 mark + 1 BONUS mark).

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Presence of self-assembled monolayers associated with extracellular matrix peptides and glycosaminoglycans to act on specifically on integrin-receptors and promoting adhesion to the surface of culture flasks. It possesses specific cell binding domains associated with fibronectin protein.

NIH-3T3 fibroblast cells predominantly use protein domains such as α5β1 integrin domain to attach to any surfaces.

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