You are interested in determining the function of a newly identified receptor tyrosine kinase (RTK) in...
You are interested in determining the function of a newly identified receptor tyrosine kinase (RTK) in cultured cells. What specific method could you utilize to determine the receptor’s function? Provide a brief description of how this method works.
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You are interested in determining the function of a newly
identified receptor tyrosine kinase (RTK) in...
You are interested in studying potential interaction of two proteins in cultured cells. What technique would...
You are interested in studying potential interaction of two proteins in cultured cells. What technique would best accomplish this goal? Include a description of how this technique works in your answer, and the possible results that would be expected (for a positive interaction or conversely, no interaction). (4 pts) You are starting a new research project and are interested in determining the function of a novel gene in cultured cells (called novl). What technique would you employ to study the...
You have just discovered a new growth factor (NEF) that binds to a tyrosine kinase receptor...
You have just discovered a new growth factor (NEF) that binds to a tyrosine kinase receptor in nerve cells. The amount of diacylglycerol in the plasma membrane increases in cells expressing this receptor when treated with treated with NEF. Propose and simple signaling pathway and identify any isoforms of possible participating enzymes. Would you expect the concentrations of any other common second messenger to increase on NEF treatment?
Select one compound (drug or toxicant) that targets one of the three receptor types(G-protein coupled receptor pathway, A receptor tyrosine kinase pathway, An ion channel pathway ) and discuss the fol...
Select one compound (drug or toxicant) that targets one of the three receptor types(G-protein coupled receptor pathway, A receptor tyrosine kinase pathway, An ion channel pathway ) and discuss the following for the selected compound: The name of the receptor involved The type of ligand that the drug or toxicant presents: Agonist, antagonist Are there natural ligands that interact with the receptor? If so explain the interaction. Provide a brief explanation of the mechanism of the cellular response upon ligand...
Research and describe a cell receptor involved in cell signaling that utilizes one of the following...
Research and describe a cell receptor involved in cell signaling that utilizes one of the following mechanisms. 1. RTK pathway (Receptor Tyrosine Kinase Cascade) 2. GPCR (G Protein Coupled Receptor) 3. Ion Channel Receptors 4. Ligand Gated Your summary of the protein receptor must include the following: 1. Name of the receptor and its ligand. 2. A brief description of the pathway (what is activated and how). 3. Name of the pathway it is responsible for activating and what the...
Drosopholia eye development is an example of cell differentiation directed by signal binding to a receptor...
Drosopholia eye development is an example of cell differentiation directed by signal binding to a receptor tyrosine kinase (RTK). In this pathway, R7 photoreceptor development requires a receptor (SEV, sevenless), which is activated by binding to a plasma membrane bound signal (BOSS, bride-of-sevenless) displayed on the adjacent R8 cell Receptor activation leads to activation of RAS via two proteins, Drk (down- stream of receptor kinases) and Sos (son-of-sevenless). Activated RAS leads to activation of a phosphorylation cascade that activates the...
The small monomeric G protein Ras is an important component of most receptor tyrosine kinase signaling...
The small monomeric G protein Ras is an important component of most receptor tyrosine kinase signaling pathways. It can regulate a number of downstream signaling components, perhaps most famously the MAPK cascade. Unlike other monomeric G proteins that we have discussed previously in class, Ras is anchored at the plasma membrane by a covalently bound lipid anchor. If Ras was specifically mutated so that it did not get this lipid modification (but was otherwise completely unchanged), what do you hypothesize...
You perform an assay on the activity of the JAK tyrosine kinase using a 40 microliter...
You
perform an assay on the activity of the JAK tyrosine kinase using a
40 microliter aliquot of a lysate prepared from cells labeled for
six minutes with radioactive cysteine. the total volume of the
lysate is 5 milliliters (ml). In the aliquot, you measure the
amount of all proteins and determine the presence of 13 micrograms
of protein in 75 microliters of lysate. which of the following is
the best calculation of the specific activity of the kinase in...
KB) You wish to determine the hormone-binding specificity of a newly identified membrane receptor. Three different...
KB) You wish to determine the hormone-binding specificity of a newly identified membrane receptor. Three different hormones, X, Y, and Z, were mixed with the receptor in separate experiments, and the percentage of binding capacity of the receptor was determined as a function of hormone concentration, as shown in graph A. 100 X BO 60 60 40 20 20 10 10 10 10 Hormone concentration (M) 10" 10" 10" 10° Hormone concentration (M) You next wish to determine whether the...
You have developed an activator of a non-receptor tyrosine kinase. In three different cell types that...
You have developed an activator of a non-receptor tyrosine kinase. In three different cell types that all contain this receptor your drug has different effects. In cell type A you see activation of gene X and gene Y; in cell type B you note activation of Gene X, but no change in the mRNA levels for Gene Y. In cell type C your drug has no effect on either Gene X or Y. Describe how could each choice (A-D) be...
You have developed an activator of a non-receptor tyrosine kinase. In three different cell types that...
You have developed an activator of a non-receptor tyrosine kinase. In three different cell types that all contain this receptor your drug mas different effects. In cell type A you see activation of gene X and gene Y; in cell type B you note activation of Gene X, but no change in the mRNA levels for Gene Y. In cell type C your drug has no effect on either Gene X or Y. Describe how could each choice (A-D) be...
You are interested in studying potential interaction of two proteins in cultured cells. What technique would best accomplish this goal? Include a description of how this technique works in your answer, and the possible results that would be expected (for a positive interaction or conversely, no interaction). (4 pts) You are starting a new research project and are interested in determining the function of a novel gene in cultured cells (called novl). What technique would you employ to study the...
Drosopholia eye development is an example of cell differentiation directed by signal binding to a receptor tyrosine kinase (RTK). In this pathway, R7 photoreceptor development requires a receptor (SEV, sevenless), which is activated by binding to a plasma membrane bound signal (BOSS, bride-of-sevenless) displayed on the adjacent R8 cell Receptor activation leads to activation of RAS via two proteins, Drk (down- stream of receptor kinases) and Sos (son-of-sevenless). Activated RAS leads to activation of a phosphorylation cascade that activates the...
The small monomeric G protein Ras is an important component of most receptor tyrosine kinase signaling pathways. It can regulate a number of downstream signaling components, perhaps most famously the MAPK cascade. Unlike other monomeric G proteins that we have discussed previously in class, Ras is anchored at the plasma membrane by a covalently bound lipid anchor. If Ras was specifically mutated so that it did not get this lipid modification (but was otherwise completely unchanged), what do you hypothesize...
You
perform an assay on the activity of the JAK tyrosine kinase using a
40 microliter aliquot of a lysate prepared from cells labeled for
six minutes with radioactive cysteine. the total volume of the
lysate is 5 milliliters (ml). In the aliquot, you measure the
amount of all proteins and determine the presence of 13 micrograms
of protein in 75 microliters of lysate. which of the following is
the best calculation of the specific activity of the kinase in...
KB) You wish to determine the hormone-binding specificity of a newly identified membrane receptor. Three different hormones, X, Y, and Z, were mixed with the receptor in separate experiments, and the percentage of binding capacity of the receptor was determined as a function of hormone concentration, as shown in graph A. 100 X BO 60 60 40 20 20 10 10 10 10 Hormone concentration (M) 10" 10" 10" 10° Hormone concentration (M) You next wish to determine whether the...
You have developed an activator of a non-receptor tyrosine kinase. In three different cell types that all contain this receptor your drug has different effects. In cell type A you see activation of gene X and gene Y; in cell type B you note activation of Gene X, but no change in the mRNA levels for Gene Y. In cell type C your drug has no effect on either Gene X or Y. Describe how could each choice (A-D) be...
You have developed an activator of a non-receptor tyrosine kinase. In three different cell types that all contain this receptor your drug mas different effects. In cell type A you see activation of gene X and gene Y; in cell type B you note activation of Gene X, but no change in the mRNA levels for Gene Y. In cell type C your drug has no effect on either Gene X or Y. Describe how could each choice (A-D) be...
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