Question

Pictures of mutant dpy-11 c. Elegans. Please tell me the characteristics of microscopic images of mutant dpy-11 c. Elegans and if possible which stage it belongs?

Below is the picture of mutant rol-6 C. elegans. Please tell me the characteristics of microscopic image of rol-6 mutant c. Elegans and if possible which stage it belongs?

Answer 1

FIRST PICTURE (dpy -11 c)- The DPY-11 protein is made exclusively in the hypodermis and resides in the cytoplasmic compartment. Whereas the TRX domain of DPY-11 displays a catalytic activity in vitro, mapping of lesions in different mutant alleles and functional analysis of deletion transgenes reveal that both this enzymatic activity and transmembrane topology are essential for determining body shape and ray morphology. Based on the abnormal features in both the expressing and non-expressing ray cells, we propose that the DPY-11 is required in the hypodermis for modification of its substrates. In turn, ray cell interaction and the whole morphogenetic process can be modulated by these substrate molecules. dpy-11 is an ortholog of human TMX4 (thioredoxin related transmembrane protein 4) ..

SECOND PICTURE (ROL 6 MUTANT C )- The rol-6 gene is one of the more than 40 loci in Caenorhabditis elegans that primarily affect organismal morphology.The rol-6 gene encodes a cuticle collagen related to human collagen alpha 1 (III) chain precursor (OMIM:120180), Both sqt-1 and rol-6 mutant phenotypes appear in L2, L3, L4, adult, and L2d stage animals, and their mRNAs are easily detectable during synthesis of each of these cuticles. However, both sqt-1 and rol-6 mutant animals also display abnormal phenotypes at the dauer stage, but no transcripts from either gene are detected during synthesis of the dauer cuticle.