Question

What are the possible genotypes of a recombinant cell after the conjugation of cultures 1 and 2?

Culture 1: F+, genotype A+B+C+

Culture 2: F-, genotype A-B-C-

Answer 1

Q) What are the possible genotypes of a recombinant cell after the conjugation of cultures 1 and 2?

Culture 1:- F+, genotype A+B+C+

Culture 2 :- F-, genotype A-B-C-

answer:

Conjugation:

i. It is defined as transfer of genetic material from one bacterium to another by direct contact

ii. During conjugation, donor bacterium contains DNA is called F-factor

iii.    Culture 1 X Culture 2

   F+, genotype A+B+C+ X F-, genotype A-B-C-

Result: F plasmid transfer from donor to recipient cells i.e., F- become F+ (culture 1 become same culture 2 will convert to F+ cells)