Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative. How would...
Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative. How would their results have differed if DNA replication was actually conservative in nature?
Homework Answers
Meselson and Stahl experiment proved thesemiconservative method of DNA replication. It means that the product DNA is composed of one old strand and one new strand. This was proved by the incorporation of radioisotope of nitrogen N15 was observed in only one of the new strands of the replicated product. If the DNA replication would be conservation, then both the strands would contain the N15 as the conservative hypothesis states that the product DNA is both new strands from 2 old DNA strands used for replication.
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Briefly describe the Meselson and Stahl experiment that
indicated that DNA replication is semiconservative. How would...
How did Meselson and Stahl distinguish between semiconservative and conservative replication in their experiment? a After...
How did Meselson and Stahl distinguish between semiconservative and conservative replication in their experiment? a After two rounds of replication, only mixed DNA molecules were present in the gradient. b After one round of replication, only mixed DNA molecules were present in the gradient. c After one round of replication, only heavy DNA molecules were present in the gradient. d After one round of replication, only mixed DNA strands were present in alkaline gradients.
The initial proposal for DNA replication is either conservative or semiconservative (not liberal). In the "most...
The initial proposal for DNA replication is either conservative or semiconservative (not liberal). In the "most beautiful experiment in biology", Meselson and Stahl proved it is one not the other. This is a seminal discovery that solidified the mechanism of DNA replication and transfer of genetic material. A) briefly describe the experiment and how the conclusion was reached; (2 points) B) What would the experimental result be if DNA replication is conservative? Describe for two rounds of replication starting with...
Draw and describe the results of the Meselson-Stahl experiments showing that DNA replication followed the Semi-conservative...
Draw and describe the results of the Meselson-Stahl experiments showing that DNA replication followed the Semi-conservative model. Draw and describe the results if DNA replication turned out to follow the Conservative model.
QUESTION 27 The Meselson and Stahl experiment was used to compare the conservative, semiconservative and dispersive...
QUESTION 27 The Meselson and Stahl experiment was used to compare the conservative, semiconservative and dispersive models of DNA replication. They cultured E. coli cells for several generations in a medium containing heavy nitrogen, 15 N. They then transferred the bacteria to a medium containing a lighter nitrogen isotope, 14 N. Samples were taken after the first round of DNA replication; another sample was taken after the second round of replication. They extracted DNA and centrifuged each sample. Explain (in...
After Watson and Crick proposed the double helix model of DNA, three models for DNA replication...
After Watson and Crick proposed the double helix model of DNA, three models for DNA replication were proposed: conservative, semiconservative and dispersive. The following figures illustrate the Meselson and Stahl experiment, which demonstrated the correctness of the semiconservative model of DNA replication N15 N14 How would the results be if the correct model was the conservative or dispersive: 1. Conservative: 20 40 60 80 Time (min.) 2. Dispersive 20 40 60 80 Time (min.)
In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (^15...
In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (^15 N) and then transferred the bacteria to a medium containing^14 N ("lighter" nitrogen) and allowed some of the the bacteria to undergo one round of replication and some bacteria to undergo two rounds of replication. They then performed density gradient centrifugation and looked to see where the DNA settled in the test tube. In the diagram below, draw bands indicating the density of the...
*BIOCHEMISTRY QUESTION ABOUT MESELSON AND STAHL* There were 3 possible models for DNA replication prior to...
*BIOCHEMISTRY QUESTION ABOUT MESELSON AND STAHL* There were 3 possible models for DNA replication prior to the Meselson and Stahl experiment, one of which was dispersive replication. a) Assuming that this is relatively uniform (each of the new chromosomes gets roughly equal amounts of the old ones) draw the peak traces for 0, 1, 2, 5 and 10 generations. b) If dispersive replication was not relatively uniform how would your answer change?
After one round of replication in 14-N media for the Meselson Stahl experiment, scientists could conclude...
After one round of replication in 14-N media for the Meselson Stahl experiment, scientists could conclude that: a) replication is semi-conservative b)replication is not conservative c)replication is not dispersive d)replication is neither dispersive nor conservative e) None of the above
please explain in details the answer I Q.9. (5 points) What result would Meselson and Stahl have obtained if the re...
please explain in details the answer
I Q.9. (5 points) What result would Meselson and Stahl have obtained if the replication of DNA were conservative? Give the expected distribution of "heavy", "light" and "hybrid" DNA molecules after 1.0 and 2.0 generations for conservative replication.
I Q.9. (5 points) What result would Meselson and Stahl have obtained if the replication of DNA were conservative? Give the expected distribution of "heavy", "light" and "hybrid" DNA molecules after 1.0 and 2.0 generations for...
In a famous experiment, Matthew Meselson and Franklin Stahl grew E.coli cells in a medium containing...
In a famous experiment, Matthew Meselson and Franklin Stahl grew E.coli cells in a medium containing only the "her isotope of nitrogen, SN. These cells were then transferred to a medium with the "light" isotope of nitrogen, "N. The results of the experiment supported the hypothesis of semiconservative replication, which was proposed by Watson and Crick. Heavy DNA ('N DNA), hybrid DNA, and light DNA (N DNA) can be separated by centrifugation. If cells containing 1SN DNA are transferred to...
The initial proposal for DNA replication is either conservative or semiconservative (not liberal). In the "most beautiful experiment in biology", Meselson and Stahl proved it is one not the other. This is a seminal discovery that solidified the mechanism of DNA replication and transfer of genetic material. A) briefly describe the experiment and how the conclusion was reached; (2 points) B) What would the experimental result be if DNA replication is conservative? Describe for two rounds of replication starting with...
QUESTION 27 The Meselson and Stahl experiment was used to compare the conservative, semiconservative and dispersive models of DNA replication. They cultured E. coli cells for several generations in a medium containing heavy nitrogen, 15 N. They then transferred the bacteria to a medium containing a lighter nitrogen isotope, 14 N. Samples were taken after the first round of DNA replication; another sample was taken after the second round of replication. They extracted DNA and centrifuged each sample. Explain (in...
After Watson and Crick proposed the double helix model of DNA, three models for DNA replication were proposed: conservative, semiconservative and dispersive. The following figures illustrate the Meselson and Stahl experiment, which demonstrated the correctness of the semiconservative model of DNA replication N15 N14 How would the results be if the correct model was the conservative or dispersive: 1. Conservative: 20 40 60 80 Time (min.) 2. Dispersive 20 40 60 80 Time (min.)
In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (^15 N) and then transferred the bacteria to a medium containing^14 N ("lighter" nitrogen) and allowed some of the the bacteria to undergo one round of replication and some bacteria to undergo two rounds of replication. They then performed density gradient centrifugation and looked to see where the DNA settled in the test tube. In the diagram below, draw bands indicating the density of the...
please explain in details the answer
I Q.9. (5 points) What result would Meselson and Stahl have obtained if the replication of DNA were conservative? Give the expected distribution of "heavy", "light" and "hybrid" DNA molecules after 1.0 and 2.0 generations for conservative replication.
I Q.9. (5 points) What result would Meselson and Stahl have obtained if the replication of DNA were conservative? Give the expected distribution of "heavy", "light" and "hybrid" DNA molecules after 1.0 and 2.0 generations for...
In a famous experiment, Matthew Meselson and Franklin Stahl grew E.coli cells in a medium containing only the "her isotope of nitrogen, SN. These cells were then transferred to a medium with the "light" isotope of nitrogen, "N. The results of the experiment supported the hypothesis of semiconservative replication, which was proposed by Watson and Crick. Heavy DNA ('N DNA), hybrid DNA, and light DNA (N DNA) can be separated by centrifugation. If cells containing 1SN DNA are transferred to...
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