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How did Meselson and Stahl distinguish between semiconservative and conservative replication in their experiment? a After...

How did Meselson and Stahl distinguish between semiconservative and conservative replication in their experiment?

a After two rounds of replication, only mixed DNA molecules were present in the gradient.

b After one round of replication, only mixed DNA molecules were present in the gradient.

c After one round of replication, only heavy DNA molecules were present in the gradient.

d After one round of replication, only mixed DNA strands were present in alkaline gradients.

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Answer #1

In Meselson and Stahl experiment they grew the bacteria continuously in N15 media then transferred to N14 media, after one round of DNA replication, they found that new Double-stranded DNA molecules had the intermediate density, this shows the DNA replication is semiconservative, one heavy strand from the parental DNA is used as the template strand so that the newly synthesized double-stranded DNA has one heavy parental strand and one new light DNA strand.

so the answer is b After one round of replication, only mixed DNA molecules were present in the gradient.

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