Homework Answers
Ans.) DFP , Serine proteases: DFP is used in biochemical studies for the blocking of serine proteases which has a molecular mechanism similar to esterases through phosphorylation and thereby is used for avoiding proteolysis in tissue homogenates.
-EDTA, Metalloenzymes: For example the reducing effect of EDTA on alkaline phosphatase of bone, intenstine and placenta of human.
-IOAc , cysteine protease.
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Relate reduction in effectiveness by the following small molecules with the specific enzyme type they affect...
First question: Which type of enzyme fron table 11-2 catalyzes the following reactions (a) 600 c=0...
First question: Which type of enzyme fron table 11-2 catalyzes the
following reactions
(a) 600 c=0 CH + NADH + H - 500- + NAD HO-- CH, COO H-CH(CH HỘI NH ADP + P 5. On the free energy diagram shown, label the intermediate(s) and transition state(s). Is the reaction thermodynamically favorable? 6. Urcase, the first enzyme to be crystallized, is inhibited in the presence of Hg, Cd, or Co ions What does this information suggest about the catalytic mechanism...
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much...
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much of their three dimensional shape is the result of interactions between the R (variable) groups of their amino acids. The active site is the portion of the enzyme that will interact with the substrate the molecule that the enzyme acts upon. The nature and arrangement of amino acids in the active site make each enzyme specific to a substrate and to the reaction it...
2. Enzymes are important biological molecules and they act as catalysts for specific chemical reactions. Their...
2. Enzymes are important biological molecules and they act as catalysts for specific chemical reactions. Their biological function is determined by their 3 dimensional shape and the functional groups present in the active site. (a) What are the main mechanisms by which an enzyme increase the rate of a reaction? (b) What is a cofactor in an enzyme mediated reaction? (c) Give the name of a cofactor (structure if possible) and indicate the type of reaction that it facilitates. 3....
For the following questions, consider six proteins, each 50 amino acids long, made of just a single type of amino acid....
For the following questions, consider six proteins, each 50
amino acids long, made of just a single type of amino acid. Protein
P is made of proline; K, lysine; S, serine; E. glutamic acid; F,
phenylalanine.; and G. glycine. Your answers are going to be P, K,
S, E, F or G.
1. Which of these proteins would move fastest on SDS-PAGE?
2. Which of these proteins would move fastest on Sephadex?
3. Which of these proteins would move closest...
Week 14- Chapter 21 Homework Problem 21.72 < 52 of 58 Review Constants| Periodic Table Part A How does a point mutation for an enzyme affect the order of amino acids in that protein? Drag the term...
Week 14- Chapter 21 Homework Problem 21.72 < 52 of 58 Review Constants| Periodic Table Part A How does a point mutation for an enzyme affect the order of amino acids in that protein? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Reset Help then the order of amino acids will change in the of the polypeptide chain If the resulting codon still codes for the same amino acid, If the...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
how does changing saline concentration affect enzyme activity? (only saline not ph) what is the hypothesis...
how
does changing saline concentration affect enzyme activity? (only
saline not ph)
what is the hypothesis
Enzyme Activity Design Investigation The compound hydrogen peroxide, Hou by product of the reaction in most living organisms. However, Hydrogen peroxide is damaging to delicate molecules inside ces As a result, nearly all organisms produce the enzyme catalase, which breaks down H,O, it is formed Potatoes are one source of catalase Catalase speeds up the breakdown of hydrogen peroxide into water and gaseoul sxygen...
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
QUESTION 1 Choose the BEST description of the following terms. voltaic cells reduction redox V A....
QUESTION 1 Choose the BEST description of the following terms. voltaic cells reduction redox V A. gain of electrons B. coating of steel with zinc C. difference in potential E between anode and cathode in voltaic cell D. spontaneous redox occurs (produces current) E. electrode where oxidation occurs F. difference in potential energy between reactants and products G. electrode that does not participate in the reaction H. movement of electrons from one substance to another anode voltage v cell potential...
Discard the answers I chose Match the following scenarios with the most specific type of transport...
Discard the answers I chose
Match the following scenarios with the most specific type of transport used. Some choices may be used more than once. A A white blood cell enguits a bacterial cell A A paramecium swims close to some debris, wraps its membrane around the debris, and pulsit inside, creating a food Vacuole D. An amoeba wraps its cell membrane around a region of dissolved nutrients in water and pulls it inward, making a small vacuole A Phagocytosis...
First question: Which type of enzyme fron table 11-2 catalyzes the
following reactions
(a) 600 c=0 CH + NADH + H - 500- + NAD HO-- CH, COO H-CH(CH HỘI NH ADP + P 5. On the free energy diagram shown, label the intermediate(s) and transition state(s). Is the reaction thermodynamically favorable? 6. Urcase, the first enzyme to be crystallized, is inhibited in the presence of Hg, Cd, or Co ions What does this information suggest about the catalytic mechanism...
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much of their three dimensional shape is the result of interactions between the R (variable) groups of their amino acids. The active site is the portion of the enzyme that will interact with the substrate the molecule that the enzyme acts upon. The nature and arrangement of amino acids in the active site make each enzyme specific to a substrate and to the reaction it...
2. Enzymes are important biological molecules and they act as catalysts for specific chemical reactions. Their biological function is determined by their 3 dimensional shape and the functional groups present in the active site. (a) What are the main mechanisms by which an enzyme increase the rate of a reaction? (b) What is a cofactor in an enzyme mediated reaction? (c) Give the name of a cofactor (structure if possible) and indicate the type of reaction that it facilitates. 3....
For the following questions, consider six proteins, each 50
amino acids long, made of just a single type of amino acid. Protein
P is made of proline; K, lysine; S, serine; E. glutamic acid; F,
phenylalanine.; and G. glycine. Your answers are going to be P, K,
S, E, F or G.
1. Which of these proteins would move fastest on SDS-PAGE?
2. Which of these proteins would move fastest on Sephadex?
3. Which of these proteins would move closest...
Week 14- Chapter 21 Homework Problem 21.72 < 52 of 58 Review Constants| Periodic Table Part A How does a point mutation for an enzyme affect the order of amino acids in that protein? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Reset Help then the order of amino acids will change in the of the polypeptide chain If the resulting codon still codes for the same amino acid, If the...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
how
does changing saline concentration affect enzyme activity? (only
saline not ph)
what is the hypothesis
Enzyme Activity Design Investigation The compound hydrogen peroxide, Hou by product of the reaction in most living organisms. However, Hydrogen peroxide is damaging to delicate molecules inside ces As a result, nearly all organisms produce the enzyme catalase, which breaks down H,O, it is formed Potatoes are one source of catalase Catalase speeds up the breakdown of hydrogen peroxide into water and gaseoul sxygen...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
QUESTION 1 Choose the BEST description of the following terms. voltaic cells reduction redox V A. gain of electrons B. coating of steel with zinc C. difference in potential E between anode and cathode in voltaic cell D. spontaneous redox occurs (produces current) E. electrode where oxidation occurs F. difference in potential energy between reactants and products G. electrode that does not participate in the reaction H. movement of electrons from one substance to another anode voltage v cell potential...
Discard the answers I chose
Match the following scenarios with the most specific type of transport used. Some choices may be used more than once. A A white blood cell enguits a bacterial cell A A paramecium swims close to some debris, wraps its membrane around the debris, and pulsit inside, creating a food Vacuole D. An amoeba wraps its cell membrane around a region of dissolved nutrients in water and pulls it inward, making a small vacuole A Phagocytosis...
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