Question

how does changing saline concentration affect enzyme activity? (only saline not ph)

what is the hypothesis

Enzyme Activity Design Investigation The compound hydrogen peroxide, Hou by product of the reaction in most living organisms. However, Hydrogen peroxide is damaging to delicate molecules inside ces As a result, nearly all organisms produce the enzyme catalase, which breaks down H,O, it is formed Potatoes are one source of catalase Catalase speeds up the breakdown of hydrogen peroxide into water and gaseoul sxygen The reaction can be detected by observing the exygen Bubbles generated 24. 0 9 H20 - Problem What are optimal /pH/seline concentration conditions for cos e s affect ensure TASK Your task is to design on investigation, conduct that investigation and analyse and interpret the data As part of your investigation you will be using the assay method below to gather your results. ASSAY METHOD (what you will do te testenryme activity 1. Cocte fer per with ryme 2. Dropped ched filer inte atest tube containing substratet As the hydrogen peroxide breaks down into water and oxygen gas the bubbles of s e lect underneath the filter paper and make it rise to the surface of the hydrogen peroxide 3.Time how long takes for the filter paper to rise to the top of your testube Start as soon as the filter paper hits the face of the solutions the test be The faster the dis the faster the greater the enzyme activity DO THE FOLLOWING THINGS TO PLAN A SUCCESSFUL AND EFFECTIVE INVESTIGATION: TESTABLE QUESTION Write a question that will be answered by your investigation. The question attempts to solve the problem HYPOTHESIS wo Make a hypothesis about how you think w e will affect the rate at which the enzyme will function Identify your independent variable, your dependent rible and controlled cibles - EXPERIMENTAL PLAN charged 1. Your group must collect Quantitative data that you will analyze and eventually make a conclusion and either accept or discord your hypothesis meski ) 2. Write a numbered procedure for your experiment that lists each step. In addition to your last procedure you must include safety precautions 3. Prepare a list of materials you will need to use in this investigation 4. Design and complete e table that will present your experiments result in an organized and easy to understand way. Usually the independent variable is placed down the first column and the dependent variable is along the top row. The table, rows and columns should be titled appropriately a lso . ) THESE NEXT STEPS ARE INCLUDED TO HELP YOU COMPLETE YOUR POST INVESTIGATION ANALYSIS RESULTS You will include your observation table and a graph of these results. DISCUSSION - Describe what did you expect to happen/see in the investigation and why. Describe what you observed. What do your results, collected data, indicate about the activity of peroxidase? Describe any trends, if applicable Canalysis] Describe why might the enzyme peroxidase behaved the way it did interpret] - Was your hypothesis valid (accurate)? Explain - Having completed the investigation, give 2 improvements or 2 future areas to investigate or 1 of each. How might you design it better the next time? What further enzyme investigation would you like to explore in the future. CONCLUSION I State the purpose of the experiment 2. State the significant findings

Answer 1

The presence of Saline/salt usually affect the conformation. folding. stability and activities of enzymes.If the salt concentration is close to zero, the charged amino acid side chains of the enzyme molecules will attract to each other. The enzyme will denature and form an inactive precipitate.Increasing salt concentration means increasing the amount of ions in solution. These ions can disrupt the electrostatic interactions between amino acids that hold the tertiary structure together. Since correct protein folding is absolutely necessary for the function of the enzyme, this can render the enzyme nonfunctional, or denature the enzyme.

Hypothesis is related to hydrogen peroxide which is a weak acid forming hydroperoxide or peroxide salts with many metals.Catalysis is an enzyme which is found almost in all living cells especially in eukaryote cells. It main function is to break down the hydrogen peroxide.Basically a cell uses catalysis to break down the hydrogen peroxide into water and oxygen.Salt concentration has to be in its intermediate state for an catalysis enzyme to work properly. For instance, if the salt concentration is too high, then the enzyme site will be blocked by the salt ions.