Question

1 of 1 Part D-A typical enzyme experiment A common enzyme studied in many introductory biology labs is catalase, which is found in high concentration in most cells, promoting the decomposition of hydrogen peroxide (H2O2) Hydrogen peroxide is formed as a by-product of chemical reactions and would soon kill cells if not immediately removed or broken down into its products--water and oxygen-as shown by the following equation Catalase 2 H2O, → 2 H2O + 0, You may be familiar with the action of catalase from pouring hydrogen peroxide on a cut, to cleanse the wound. The bubbles formed are the oxygen produced from the breakdown of hydrogen peroxide by the catalase in your body's cells Catalase can be obtained from many sources, such as potatoes, liver, and yeast. In this lab, you will add catalase to a reaction chamber filled with hydrogen peroxide and measure the oxygen produced You will then use this measurement to calculate the reaction rate. This simple procedure can be easily adapted to investigate numerous questions about enzyme activity. Your instructor may select a different enzyme/substrate pair or procedure for your investigation, but the steps that follow illustrate one technique. Just prior to running this investigation, prepare a catalase solution by adding 1 package (7g) yeast to 200 ml warm water and stir to dissolve. Set up the experiment as described in the figure below Ring stand 100 ml graduated linder filled with water Reaction chamber (with H/catalise solution Stopoer Via Pipette JUODOW) O, bubbles Fill a plastic storage box with water Filla graduated cylinder with water and invertit so that its opening suspended in the water. The cylinder can be held in place using a ring stand, or a member of the team boltinn Prepare a reaction chamber by fitting a small container such as a frutfly van with a one hole stopper. Insert a short piece of a snug-litong plastic pipette into the hole. Pour 10 ml of hydrogen peroxide H,0) into the them Submerge the reaction chamber in the water bath, placing it so that all the bubbles formed in the reacton chamber are captured by the inverted graduated cyinder

Answer 1

The correct option is

C) The level of oxygen in the cylinder will remain constant.

Hydrogen peroxide is the substrate for catalase enzyme to produce oxygen and water. Oxygen is a gas and therefore will escape the liquid as bubbles. When there is no hydrogen peroxide present, the catalase will not function, and oxygen production will be stopped. So, the level of oxygen in the cylinder will remain constant. The enzymes remain unchanged during and after a reaction.