Why were your cheek cell sample boiled for 10 minutes? Why was chelex included?
Why were your cheek cell sample boiled for 10 minutes?
Why was chelex included?
Homework Answers
As far as I know the process mentioned in the question is for DNA extraction.
A) The sample are boiled to disrupt the cell and nuclear membrane of the cell so that the DNA that is present in the nucleus can come out of the cell and is available for purification.
B Chelex is a chelating agent that stops the activity of Mg2+ ions that are needed for DNAse activity, thus reducing the chance of degradation of genetic material by DNAse. This is added so that the sample can be used in future as a PCR template.
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Why were your cheek cell sample boiled for 10 minutes?
Why was chelex included?
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54 Use information on your Summary Chart (page 31) to eui mate the diameter of cheek epithelial cells. Record the total magnification (TM) used. total n Why do your cheek cells look different from those illustrated in Figure 3.5? (Hint: What did you have to do to your choek to obtain them?) mount, dispose of your wet mount p n in the beaker of bleach solution, and put the coverslips in an auto- clave bag. Figure 3.5 Epithelial cells of...
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