You extracted your DNA from cheek cells. The concentration of your DNA sample is 250...
You extracted your DNA from cheek cells. The concentration of your DNA sample is 250 ng/?l. The DNA template for PCR should be 10 ng/?l. You should mix _____ ?l of your DNA sample and _____ ?l of water to make final volume of 100 ?l of DNA that has the concentration of 10 ng/?l.
?
Homework Answers
Volume of stock to add =
required final conc of DNA * final volume / actual concn of stock DNA
= 10 * 100 / 250
= 4 microl
4 microl of stock sna should be mixed with 96 microl of water to make solution of 10 ng/microl.
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You extracted your DNA from cheek cells. The
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How to solve for question#6?
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