Homework Answers
Yes, there is little bit difference between the method to extract the DNA from plant and animal cells. The steps common to both method is treatment plant and animal cells with a soapy substance, it will degrade the lipids in the cell and nuclear membranes. Then, the DNA mixture will separate from the cell membranes and proteins. Next, you can use alcohol to precipitate the DNA in the solution. Depending on the amount in the sample, DNA may be visible with the naked eye. However, keep in mind that this simple procedure does not necessarily produce DNA of high purity.
Additional step in plant cells DNA extraction is addition of cellulase or homozenization to remove the rigid cell wall.
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Dind Less Based on the method used to extract DNA from your cheek cells, are there changes to the...
You extracted your DNA from cheek cells. The concentration of your DNA sample is 250...
You extracted your DNA from cheek cells. The concentration of your DNA sample is 250 ng/?l. The DNA template for PCR should be 10 ng/?l. You should mix _____ ?l of your DNA sample and _____ ?l of water to make final volume of 100 ?l of DNA that has the concentration of 10 ng/?l. ?
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
Suppose you were able to choose three fossils to extract DNA from and your goal was...
Suppose you were able to choose three fossils to extract DNA from and your goal was to test the hypothesis that completely modern humans evolved in Africa. Which three would you pick? Explain why. Important information: This discussion is to focus on MODERN humans. That means hominids that appeared in the fossil record after 300kya. Please focus on the topic. Consider how you would use DNA to test the origins of modern humans. No discussion of apes, australopithecines and earlier...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer b Acidic lysis buffer RNase C d None of the above 8 What solution is used to isolate the DNA from the spin column? DNA buffer a. b. Elution buffer P1 c. d. P2 9 EcoRI restriction site is 5' GAATTC 3' and 3' CTTAAG 5 a 5' GAATTC 3' and 3' CTTAAG 5 b 5' GAATTC 3' and 3' CTTAAG 5 C. d....
2. What cellular structures must be disrupted in order to release DNA from (a) strawberry or...
2. What cellular structures must be disrupted in order to release DNA from (a) strawberry or banana cells (b) bacterial cells and (c) human tissue? (Hint: Think about what you learned about cell structure in Lesson 3, 'A Tour of the Cell.) RESPONSE: 3. If shampoo and detergent can be used to extract DNA, why don't we lose DNA from our cells every time we shower or wash our hands? RESPONSE: 4. Consider the appearance of your extracted DNA and...
DNA Extraction Worksheet - PreLab Name Class & Section DNA Extraction Worksheet - PreLab After reading...
DNA Extraction Worksheet - PreLab
Name Class & Section DNA Extraction Worksheet - PreLab After reading "Exercise 1 DDNA Extraction" in the lab manual, answer the following questions before coming to lab. This worksheet is to be turned in at the beginning of lab. 1. (2pt) Briefly, outline the procedure for isolating DNA from your cheek cells. 2. Do all cells (except mature RBCs and gametes) contain the same DNA? 3. Do all cells use genes in the same way?...
5 ul (10ng.ul) of ptud plasmid control, 2 colonies grown 100ng of DNA was used for...
5 ul (10ng.ul) of ptud plasmid control, 2 colonies grown 100ng of DNA was used for ligation, 25ng DNA was used for transformation, 1ml was the total volume of cells grown, 100 ul was plated, 130 colonies were grown 1. What was your transformation efficiency for control DNA pTUD at concentration of 10 ng/ul)? 2. What was transformation efficiency for experimental ligation (you will need to calculate approximate concentration from the steps of the cloning procedure you performed)?
54 Use information on your Summary Chart (page 31) to eui mate the diameter of cheek...
54 Use information on your Summary Chart (page 31) to eui mate the diameter of cheek epithelial cells. Record the total magnification (TM) used. total n Why do your cheek cells look different from those illustrated in Figure 3.5? (Hint: What did you have to do to your choek to obtain them?) mount, dispose of your wet mount p n in the beaker of bleach solution, and put the coverslips in an auto- clave bag. Figure 3.5 Epithelial cells of...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA out of each sample. Describe the technologies used to get the below results. 4) Identify which suspect is at the crime scene 5) Among suspect 1-3, who is homozygous for the tested locus and who is heterozygous? DNA from crime scene DNA ladder suspect DNA #1 #2 #3 500 bp 400 bp 300 bp 200 bp 100 bp — I 4. PCR Practice two...
1. Describe the changes in your potato sample when placed in distilled water. Based on these...
1. Describe the changes in your potato sample when placed in distilled water. Based on these changes, was the water hypertonic, isotonic, or hypotonic compared to the cells of the potato? Explain your reasoning in terms of the flow of water into or out of the potato cells. 2, Describe the changes in your potato sample when placed in 0.9% NaCl solution. Based on these changes, was the 0.9% NaCl hypertonic, isotonic, or hypotonic compared to the cells of the...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
Suppose you were able to choose three fossils to extract DNA from and your goal was to test the hypothesis that completely modern humans evolved in Africa. Which three would you pick? Explain why. Important information: This discussion is to focus on MODERN humans. That means hominids that appeared in the fossil record after 300kya. Please focus on the topic. Consider how you would use DNA to test the origins of modern humans. No discussion of apes, australopithecines and earlier...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer b Acidic lysis buffer RNase C d None of the above 8 What solution is used to isolate the DNA from the spin column? DNA buffer a. b. Elution buffer P1 c. d. P2 9 EcoRI restriction site is 5' GAATTC 3' and 3' CTTAAG 5 a 5' GAATTC 3' and 3' CTTAAG 5 b 5' GAATTC 3' and 3' CTTAAG 5 C. d....
2. What cellular structures must be disrupted in order to release DNA from (a) strawberry or banana cells (b) bacterial cells and (c) human tissue? (Hint: Think about what you learned about cell structure in Lesson 3, 'A Tour of the Cell.) RESPONSE: 3. If shampoo and detergent can be used to extract DNA, why don't we lose DNA from our cells every time we shower or wash our hands? RESPONSE: 4. Consider the appearance of your extracted DNA and...
DNA Extraction Worksheet - PreLab
Name Class & Section DNA Extraction Worksheet - PreLab After reading "Exercise 1 DDNA Extraction" in the lab manual, answer the following questions before coming to lab. This worksheet is to be turned in at the beginning of lab. 1. (2pt) Briefly, outline the procedure for isolating DNA from your cheek cells. 2. Do all cells (except mature RBCs and gametes) contain the same DNA? 3. Do all cells use genes in the same way?...
54 Use information on your Summary Chart (page 31) to eui mate the diameter of cheek epithelial cells. Record the total magnification (TM) used. total n Why do your cheek cells look different from those illustrated in Figure 3.5? (Hint: What did you have to do to your choek to obtain them?) mount, dispose of your wet mount p n in the beaker of bleach solution, and put the coverslips in an auto- clave bag. Figure 3.5 Epithelial cells of...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA out of each sample. Describe the technologies used to get the below results. 4) Identify which suspect is at the crime scene 5) Among suspect 1-3, who is homozygous for the tested locus and who is heterozygous? DNA from crime scene DNA ladder suspect DNA #1 #2 #3 500 bp 400 bp 300 bp 200 bp 100 bp — I 4. PCR Practice two...
1. Describe the changes in your potato sample when placed in distilled water. Based on these changes, was the water hypertonic, isotonic, or hypotonic compared to the cells of the potato? Explain your reasoning in terms of the flow of water into or out of the potato cells. 2, Describe the changes in your potato sample when placed in 0.9% NaCl solution. Based on these changes, was the 0.9% NaCl hypertonic, isotonic, or hypotonic compared to the cells of the...
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