Homework Answers
Experimental results have shown that the fusion of RS domain to Sxl (RS-Sx) retained the function of Sxl+. RS-Sx results in the female specific splicing of tra pre-mRNA. Male flies with RS-Sx transgene show male specific lethality due to the function of Sxl protein. Female flies with RS-Sx transgene are normal.
Reference:
Granadino, B., Penalva, L., Green, M., Valcarcel, J. and Sanchez, L. (1997). Distinct mechanisms of splicing regulation in vivo by the Drosophila protein Sex-lethal. Proceedings of the National Academy of Sciences, 94(14), pp.7343-7348.
Add Answer to:
CRISPR-Cas9 technology is used to engineer Drosophila melanogaster in which the Sx1 protein contains an RS...
the following scenarios require application of CRISPR-cas9 technology. Identify which technologies are the best fit for...
the following scenarios require application of CRISPR-cas9
technology. Identify which technologies are the best fit for each
research scenario
Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
regio he home or Notch he he X chror on mutation is a deletion on the...
regio he home or Notch he he X chror on mutation is a deletion on the X chromosome of Drosophila melanogaster omale flies heterozygous for Notch have an indentation on the margins of the wing och is lethal in the homozvous and hemiyvous conditions. The Notch deletion ne region of the X chromosome that contains the locus for white eyes, an X- u recessive trait. Give the phenotypes and proportions of progeny produced in the following crosses: (a) A red-eyed,...
25 10. Review each of the following scenarios and indicate which statistical analysis would be most...
25 10. Review each of the following scenarios and indicate which statistical analysis would be most appropriate to test that scenario. Explain your choice. Choose from the following statistics (Each Only Used Once): Dependent-Samples t Test Independent-Samples Test Dependent non-parametric Independent non-parametric a.Children (mean age 6 years) who ate breakfast at school participated in an experiment to determine the effect of sugar on activity. On some days a particular child received sugar with breakfast and on other days the child...
the following scenarios require application of CRISPR-cas9
technology. Identify which technologies are the best fit for each
research scenario
Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
Question 7 (1 point) CRISPR/Cas9 is often used to make mutations in zebrafish. Which statement is most correct? Capping the sgRNA is essential for its stability. The Cas9 mRNA is is used to target the native Cas9 protein to the locus to be mutated. the sgRNA uses DICER to cut up a the twenty nucleotide target region of the DNA, starting at the 3-prime end. O neomycin is used to select the cells that inherit the Cas9 insertion. O the...
regio he home or Notch he he X chror on mutation is a deletion on the X chromosome of Drosophila melanogaster omale flies heterozygous for Notch have an indentation on the margins of the wing och is lethal in the homozvous and hemiyvous conditions. The Notch deletion ne region of the X chromosome that contains the locus for white eyes, an X- u recessive trait. Give the phenotypes and proportions of progeny produced in the following crosses: (a) A red-eyed,...
25 10. Review each of the following scenarios and indicate which statistical analysis would be most appropriate to test that scenario. Explain your choice. Choose from the following statistics (Each Only Used Once): Dependent-Samples t Test Independent-Samples Test Dependent non-parametric Independent non-parametric a.Children (mean age 6 years) who ate breakfast at school participated in an experiment to determine the effect of sugar on activity. On some days a particular child received sugar with breakfast and on other days the child...
Most questions answered within 3 hours.
-
Describe how to distinguish true motility from brownian motion
and fluid flow when viewing a wet...
asked 19 seconds from now -
A manufacturer of game controllers is concerned that its
controller may be difficult for left-handed users....
asked 1 minute ago -
Water at gauge pressure of 3.8 atmospheres at street level
enters an office building at 0.60...
asked 15 minutes ago -
Quick question, I want to know how to convert decimal numbers to
binary number, when i...
asked 36 minutes ago -
You found out that now you are going to receive payments of
$8,500 for the next...
asked 40 minutes ago -
17.
Technician A says that leaking components are usually repaired
rather than replaced. Technician B says...
asked 41 minutes ago -
A) An Operon is:?
1- A gene that affect another gene
transcription.
2. A gene coding...
asked 47 minutes ago -
When opening a file, use this method to determine whether a file
exists before you attempt...
asked 52 minutes ago -
Sales total $300,000 when variable costs total $180,000 and
fixed costs are $60,000. Breakeven sales total:...
asked 58 minutes ago -
1)
To calculate the depth of a water well, an explorer drops a stone and with...
asked 56 minutes ago -
0.429-g sample of gas occupies 125 mL at 60. cm of Hg and 25°C.
The molar...
asked 58 minutes ago -
The
pH of a 0.90M solution of boric acid (H3BO3) is measured to be
4.64.
Calculate...
asked 1 hour ago