If you want to change the buffer composition of a protein solution via diafiltration, how many...
If you want to change the buffer composition of a protein solution via diafiltration, how many reservoir volumes are required to remove 99% of the original buffer components?
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If you want to change the buffer composition of a protein
solution via diafiltration, how many...
a) You want to load 15 ug of protein in 10 uL into one of the...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
4. If you want to make a buffer of 4.5 pH, how many grams of NaF...
4. If you want to make a buffer of 4.5 pH, how many grams of NaF do you need to add to 300 mL of 0.4 M HF solution? (Ka=7.1x10-4) Answer: 114.66 grams Pka -log (7.1 X10-4) = 3.1487411651 234
Buffer Practice 1.) You wish to prepare 750 mL of a buffer solution of pH =...
Buffer Practice 1.) You wish to prepare 750 mL of a buffer solution of pH = 7.00 and you decide to use NaH2PO4 as the acid, K2 = 6.2 x 10. You have on hand a 0.175 M solution of this acid. Kai = 7.5 x 103, Ka2 = 6.2 x 10-8, K3 = 4.2 x 10-13 a.) Calculate the pH of the NaH2PO4 solution. b.) Calculate the grams of Na2HPO4 that need to be added to 750 mL of...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol) Urea (0.075 M, 60.06 g/mol) NaClI (150 mM, FW 58.44 g/mol) Dithiothreitol (4 mM, MW = 154.25 g/mol) B-Mercaptoethanol (50 mM, MW 78.13 g/mol) You need to make 2500 mL of this buffer. In your lab you have a bottle of 500 mM Tris-HCl, 125 mM β-mercaptoethanol, and solid urea, NaCl, and dithiothreitol. Calculate the amount of each...
You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular...
You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular weight of 22,680 g/mol is 75 µM. What is the concentration in mg/ml?
How are we getting the volumes in this solution for example for 10Xs buffer how did...
How are we getting the volumes in this solution for example
for 10Xs buffer how did we get 1 microliter please explain for each
reagent how the microliters were obtained; ddH20, 10X buffer A, 1
microgram/microliter DNA stock, and enzyme at 10
U/microliter.
Question: What is the most concentrated stock of buffer A you can make? Now if you want to prepare an enzymatic reaction containing 1X buffer A, 100 ng/ul DNA stock and 1 Unit/ul (U/ul) of enzyme proceed...
In lab, you diluted your unknown protein solution by combining 14 ?L of unknown protein solution...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
Titration of the carbonate buffer system lab 1. How would you expect buffer capacities to change...
Titration of the carbonate buffer system lab 1. How would you expect buffer capacities to change if larger masses of sodium carbonate and sodium bicarbonate were used in each titration? 2. How would you expect the buffer capacities to change if a more concentrated solution of HCl was used in the titration?
(3 pts) 4. You are asked to prepare a 0.100 M)glycine buffer pH 9.0. You are given a bottle of glycine (FW 75.1 g/mol) and a stock solution of 1.00 M NaOH. How many grams of glycine and how many...
(3 pts) 4. You are asked to prepare a 0.100 M)glycine buffer pH 9.0. You are given a bottle of glycine (FW 75.1 g/mol) and a stock solution of 1.00 M NaOH. How many grams of glycine and how many milliliters of NaOH stock solution would be required to produce 2 liters of this buffer solution? pk,95 alyeine tr oon 2 38 CHA) 7 3A1 (HA o.l0o glycine 3.98
(3 pts) 4. You are asked to prepare a 0.100 M)glycine...
How many moles of NH3 are required in a 1.00L solution to create a buffer with...
How many moles of NH3 are required in a 1.00L solution to create a buffer with a pH of 8.81 if the solution contains 0.64 moles of NH4Cl? Kb NH3 = 1.8 x 10-5?
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
4. If you want to make a buffer of 4.5 pH, how many grams of NaF do you need to add to 300 mL of 0.4 M HF solution? (Ka=7.1x10-4) Answer: 114.66 grams Pka -log (7.1 X10-4) = 3.1487411651 234
Buffer Practice 1.) You wish to prepare 750 mL of a buffer solution of pH = 7.00 and you decide to use NaH2PO4 as the acid, K2 = 6.2 x 10. You have on hand a 0.175 M solution of this acid. Kai = 7.5 x 103, Ka2 = 6.2 x 10-8, K3 = 4.2 x 10-13 a.) Calculate the pH of the NaH2PO4 solution. b.) Calculate the grams of Na2HPO4 that need to be added to 750 mL of...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol) Urea (0.075 M, 60.06 g/mol) NaClI (150 mM, FW 58.44 g/mol) Dithiothreitol (4 mM, MW = 154.25 g/mol) B-Mercaptoethanol (50 mM, MW 78.13 g/mol) You need to make 2500 mL of this buffer. In your lab you have a bottle of 500 mM Tris-HCl, 125 mM β-mercaptoethanol, and solid urea, NaCl, and dithiothreitol. Calculate the amount of each...
How are we getting the volumes in this solution for example
for 10Xs buffer how did we get 1 microliter please explain for each
reagent how the microliters were obtained; ddH20, 10X buffer A, 1
microgram/microliter DNA stock, and enzyme at 10
U/microliter.
Question: What is the most concentrated stock of buffer A you can make? Now if you want to prepare an enzymatic reaction containing 1X buffer A, 100 ng/ul DNA stock and 1 Unit/ul (U/ul) of enzyme proceed...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
(3 pts) 4. You are asked to prepare a 0.100 M)glycine buffer pH 9.0. You are given a bottle of glycine (FW 75.1 g/mol) and a stock solution of 1.00 M NaOH. How many grams of glycine and how many milliliters of NaOH stock solution would be required to produce 2 liters of this buffer solution? pk,95 alyeine tr oon 2 38 CHA) 7 3A1 (HA o.l0o glycine 3.98
(3 pts) 4. You are asked to prepare a 0.100 M)glycine...
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