Question

3.) A series of chromatographic peaks are referred to as a chromatogram. a.) Describe the general shape of a chromatographic peak? b.) How does the term resolution relate to a chromatographic separation? Include as part of your answer the two factors that determine resolution? 4.) List the five basic components of all liquid chromatograph systems

Answer 1

A) 3 ) Signal Intensity time and This is a general chromatogram. et is Showing Signal intensity from detector with a function of retention time, joten b) Resolution : 24 measures the separation - between two peaks of different retention time. leak resolution = 2(tz - til ولي + ولي / و where tz and t, represents retention time of two different peaks ehf w, and wil is the peak width out baseline Resolution depends on many other factors & Temperature and Solvert. column type carrier gas in Gas Chromatography,

Five basic components of all Liquid cheomatogeaphy Systems . Detector Injector Pump computer column liquid chromatography separation is based on absorption phenomena. Selection of mobile phase and stationary I phase is very important.