Question

1. The outer leaflet of the outer membrane is composed of _______ and _______.

		Di glucosamine which has a hydrophilic characteristic; myristic acid which has a hydrophilic characteristic
		N-acetyl glucosamine which has a hydrophobic characteristic; myristic acid which has a hydrophilic characteristic
		Di glucosamine which has a hydrophobic characteristic; myristic acid which has a hydrophobic characteristic
		Di glucosamine which has a hydrophillic characteristic; myristic acid which has a hydrophobic characteristic
		N-acetyl glucosamine which has a hydrophillic characteristic; myristic acid which has a hydrophobic characteristic 2. "In an osmotic shock experiment, the cells need to be sequentially treated in differing osmotic pressure fluids. Identify the correct combination and sequence of treatments with the appropriate bacterium below." Salmonella typhi; water; 40% sucrose Salmonella typhi; 40% sucrose; water "Streptococcus mutans, 40% sucrose; water" None of the above

Answer 1

1. Option 4 is the answer

Myristic acid is a 14 carbon compound unsaturated fatty acid which is hydrophobic and is found in less concentration in cell membrane. D glucosamine is hydrophilic in nature and is positive charged , gives membrane protection and membrane stability.

2. None of the above

E.coli is the model organism used for conducting osmotic shock experiment. The experiment is conducted in two stages.

Stage I cells treated with 0.03mm tris pH 7.3, 10 raise to -4 M EDTA , 20 percent sucrose for 20 minutes.

Stage II : Cells rapidly dispersed in 5* 10 raise to -4 Molar MgCl2.