How does Cas9 get “told” where to cut in the genome? Explain.
Copy of In the CRISPR/Cas9 genome editing system, the job of the guide RNA is to... o cut the target sequence The job of the guide RNA is not fully known yet. o identify the correct sequence to be cut o help the plasmid enter the cells hide the plasmid from the immune system
How and where does the HSV-1 genome exist in a host cell? Integrated into the host cell chromosome In the cytoplasm as a linear chromosome. O In the cytoplasm as a circular episome In the nucleus as a circular episome
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural function of this system? Describe how you would use this system to generate a null mutation in another organism (i.e. explain Figure 6-43). How does it work? What is the modification of the method that allows for correction of a mutation (e.g. the mouse crystalline gene)? And lastly, what are the problems with the CRISPR system? FIGURE 6-43 Single-nucleotide mutations can be introduced into...
I was told to start with a piece of printer paper and cut it in half. Set aside one of the halves. Then cut the second half in half again. Repeat this until you are unable to cut the paper. The results were 13 cuts and a final area of 7.36E-6 m^2. Each cut represents a half-life of a molecule. Please explain how this relates to the half-life of Uranium-235?
Can someone explain how to get from previous step to this step? Thank you! where does the 3^4 come from? = (yཀ=u(na + 2) - (yཀལ* (n + 1) 3*6(n + 2) – 545 (n + 1) – 180,"u(n)
How many times do the enzymes BamHI and EcoRI cut the S. cerevisiae genome within the region spanned by the probe? I am very confused and don't understand this question? Can someone plz EXPLAIN it to me A Southern hybridization was performed on genomic digests of Saccharomyces cerevisiae. Following migration on an agarose gel, the digests were probed with a 3Kbp radioactive probe. The results obtained following the hybridization are presented below Size of hybrids (Kbp Enzyme BamHI EcoRI EcoRI+...
Discuss Genome characteristics – explain how genome size and gene density differ in simple organisms, such as prokaryotes, versus more complex organisms, such as ourselves. Discuss why these different genome characteristics exist in simple versus complex organisms.
the following scenarios require application of CRISPR-cas9 technology. Identify which technologies are the best fit for each research scenario Cas9 and HDR 1. Researchers want to make an endonuclease that binds to target DNA, but does not cut the DNA. This will allow them to target specific areas of the genome for other modifications without making cuts. dCas9 dCas9 and repression domain 2. Researchers are studying a proposed oncogene in mice. They want to examine what happens when the gene...
Where does a protein with a sorting signal get translated? Where does a protein without a sorting signal get translated?
Can somone please explain how to graph this, please show work and explain where you get the numbers. Thank you Draw the that z 0 m at t = 08.(Figuro 1) graph for the velocity 1 o1 No elements selected r(m) y, (m/s) t (s) 10 12