Question

Biochemistry inhibitors! Pls answer 1 through 11

For each of the following items, indicate whether the item pertains to: • Competitive inhibitor . Uncompetitive inhibitor • Noncompetitive inhibitor • Noncompetitive activator • None of the above Choose only one of the above for each item. 1. Binds to the enzyme-substrate complex only 2. Prevents substrate from binding enzyme 3. Forms inactive El or inactive ESI complex 4. When present, Vmax increases 5. When present, Vmax increases and Km decreases 6. When present, Vmax of the enzyme is unaffected 7. On a Lineweaver-Burke plot, the Y intercept moves closer to the origin but the Km remains unchanged 8. On a Lineweaver-Burke plot, the Y intercept moves away from the origin but the Km remains unchanged 9. On a Lineweaver-Burke plot, the Y intercept and the X intercept both move closer to the origin 10. Structurally similar to the substrate 11. Describes the effect of the PKA regulatory subunit on the PKA catalytic subunit

Answer 1

1. Uncompetitive inhibitor. An uncompetitive inhibitor binds at a distinct site from active site, but only binds with enzyme substrate complex.
2. Competitive inhibitor. A competitive inhibitor binds with enzyme's active site and thus inhibitor compete with substrate for binding site.
3. Uncompetitive inhibitor. Inhibitor binds with enzyme substrate complex and forms enzyme substrate inhibitor complex.
4. None of the above. In case of competitive inhibitor Vmax remains unchanged whereas Vmax decreases in case of uncompetitive and noncompetitive inhibitiors.