1. If you add short actin filaments marked by bound myosin heads (myosin-decorated filaments) to a...
1. If you add short actin filaments marked by bound myosin heads (myosin-decorated filaments) to a solution with an excess of actin monomers, wait for a few minutes, and then examine the filaments by electron microscopy, you see the picture shown in Figure 16-5.
A. Which is the plus end of the myosin-decorated filaments and which is the minus end? Which is the "barbed" end and which is the "pointed" end? How can you tell?
B. If you diluted the mixture so that the actin concentration was below the critical concentration, which end would be depolymerize more rapidly?
C. When the actin filament depolymerizes, why are subunits removed exclusively from the ends and not from the middle of the filament?
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1. If you add short actin filaments marked by bound myosin heads
(myosin-decorated filaments) to a...
biochem help plz. The balance between the association of actin monomers to form a filament and...
biochem help plz.
The balance between the association of actin monomers to form a filament and dissociation of monomers from the actin filament is a Myosin independent b. Myosin dependent c. ATP independent d.ATP dependent e. charge dependent QUESTION 2 Which statement about actin is correct? a Actin is expressed only in muscle b. Actin is expressed at low levels in most cells. GNo known function has been described for actin in nonmuscle cells. d. Nonmuscle cells only contain G...
An explanation would be appreciated. Thank you! Compared to the normal situation, in which actin monomers...
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
Isolated bundles of actin filaments from the acrosomal processes of Limuluspolyphemus(horseshoe crab) sperm have readily distinguishable...
Isolated bundles of actin filaments from the acrosomal processes
of Limuluspolyphemus(horseshoe crab) sperm have readily
distinguishable plus ends (tapered) and minus ends
(blunt). Assembly at the ends of such bundles was used
to determine the mechanism of action of phalloidin, which has a
marked effect on actin assembly. When phalloidin is mixed with
actin in a molar ratio of at least 1:1, the growth rate increases
at both ends, as shown for minus ends in Fig.
16-11A. Because growth rate = kon[actin]...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
biochem help plz.
The balance between the association of actin monomers to form a filament and dissociation of monomers from the actin filament is a Myosin independent b. Myosin dependent c. ATP independent d.ATP dependent e. charge dependent QUESTION 2 Which statement about actin is correct? a Actin is expressed only in muscle b. Actin is expressed at low levels in most cells. GNo known function has been described for actin in nonmuscle cells. d. Nonmuscle cells only contain G...
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
Isolated bundles of actin filaments from the acrosomal processes
of Limuluspolyphemus(horseshoe crab) sperm have readily
distinguishable plus ends (tapered) and minus ends
(blunt). Assembly at the ends of such bundles was used
to determine the mechanism of action of phalloidin, which has a
marked effect on actin assembly. When phalloidin is mixed with
actin in a molar ratio of at least 1:1, the growth rate increases
at both ends, as shown for minus ends in Fig.
16-11A. Because growth rate = kon[actin]...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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