step by step procedure to detect the presence of nitrate reductase gene in a bacterial sample
step by step procedure to detect the presence of nitrate reductase gene in a bacterial sample
Homework Answers
- Collection of bacterial samples
- Maintaining the bacterial strains under proper growth conditions
- Then the bacterial DNA is isolated
- PCR amplification of nitrate reductase gene is done
- Phylogenetic analysis of nitrite reductase genes is done
- T-RELP analysis of nitrite reductase genes
Add Answer to:
step by step procedure to detect the presence of nitrate
reductase gene in a bacterial sample
Design a detailed, step-by-step, experiment to a) Detect the presence of Nitrate reductase gene in your...
Design a detailed, step-by-step, experiment to a) Detect the presence of Nitrate reductase gene in your unknown bacterial sample b) Detect the presence of Nitrate reductase enzyme in your unknown bacterial sample
Explain the effect of the presence of potassium nitrate and sodium nitrate on the solubility of...
Explain the effect of the presence of potassium nitrate and sodium nitrate on the solubility of KHT using Le Chatelier's principle. (Begin by writing the chemical equation for the equilibrium. Consider only the magnitude of the solublility.) A laboratory experiment designed to determine the solubility product constant for magnesium hydroxide involved a similar procedure to establish a saturated solution. The hydroxide ion concentration was then determined using a pH meter. The pH was recorded as 10.52. Determine Ksp.
When and how does the RT-PCR test detect the presence of the virus? During PCR. by...
When and how does the RT-PCR test detect the presence of the virus? During PCR. by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding fluorescent primers that are detected through a special laser. After PCR, by adding a quencher dye which...
How would you explain the presence of bacterial genes being present on the genome of their...
How would you explain the presence of bacterial genes being present on the genome of their eukaryotic host, as observed in mealybugs? horizontal gene transfer random frameshift mutation intragenomic recombination recent common ancestry
Lastly, one method used to detect the presence and quantity of lead ions in water involves...
Lastly, one method used to detect the presence and quantity of lead ions in water involves adding sodium chromate to the water. The reaction produces a colored precipitate. a. Write a reaction showing how aqueous lead (II) nitrate reacts with sodium chromate. You will need to research the formula for sodium chromate and the solubility of the products. b. Research and then give the color and formula of the colored product formed. c. Explain how the production of a colored...
D Question 3 2 pts When and how does the RT-PCR test detect the presence of...
D Question 3 2 pts When and how does the RT-PCR test detect the presence of the virus? After PCR, by adding a quencher dye which is activated when the viral DNA is available in high concentrations During PCR by adding fluorescent primers that are detected through a special laser After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding a fluorescent dye and a quencher dye...
please include all the information and step by step procedure. #2: Gene Translation How many ribosomes...
please include all the information and step by step
procedure.
#2: Gene Translation How many ribosomes are actively synthesizing proteins at any instant in an E. coli cell growing with a doubling time of 45-minutes? Use the following assumptions in your calculations- a. The birth size (size just after mitosis is complete) of E. coli is 1 micron in diameter and 2 microns in length. b. Water content of the cell is 85%. 50% of the dry cell mass is...
a. Which laboratory procedure would a laboratory technician use to determine the presence of a particular...
a. Which laboratory procedure would a laboratory technician use to determine the presence of a particular drug present in small quantities from a serum sample? Discuss the principle of the procedure in full. (10) b. Describe a procedure that one would use to construct a genomic library from DNA. (10)
Your friend performed gram staining procedure on his bacterial sample, but he forgot to wash his...
Your friend performed gram staining procedure on his bacterial sample, but he forgot to wash his slide with 95% alcohol solution. What result would you expect for his sample and is it reliable? explain B 1 ol Certain diseases such as ALS affects motor neurons (neurons that voluntarily sends impulses) rendering them weak and gradually destroying these neurons. Patients having such diseases suffer from difficulty in walking, speaking, holding up posture, breathing, swallowing, and other symptoms. Explain why they would...
Hy 1.008 664,427 0 15.964 18. A biochemical engineer isolates a bacterial gene fragment and dissolves...
Hy 1.008 664,427 0 15.964 18. A biochemical engineer isolates a bacterial gene fragment and dissolves a 20.6. mg sample of the material in enough water to make 41.5 mL of solution. The osmotic pressure of the solution is 0.340 torr at 25.0°C. (a) What is the molar mass of the gene fragment (5 pts)? 2mci we6 .0206 s .0415 C= .0206 ms.
Explain the effect of the presence of potassium nitrate and sodium nitrate on the solubility of KHT using Le Chatelier's principle. (Begin by writing the chemical equation for the equilibrium. Consider only the magnitude of the solublility.) A laboratory experiment designed to determine the solubility product constant for magnesium hydroxide involved a similar procedure to establish a saturated solution. The hydroxide ion concentration was then determined using a pH meter. The pH was recorded as 10.52. Determine Ksp.
When and how does the RT-PCR test detect the presence of the virus? During PCR. by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding fluorescent primers that are detected through a special laser. After PCR, by adding a quencher dye which...
How would you explain the presence of bacterial genes being present on the genome of their eukaryotic host, as observed in mealybugs? horizontal gene transfer random frameshift mutation intragenomic recombination recent common ancestry
D Question 3 2 pts When and how does the RT-PCR test detect the presence of the virus? After PCR, by adding a quencher dye which is activated when the viral DNA is available in high concentrations During PCR by adding fluorescent primers that are detected through a special laser After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding a fluorescent dye and a quencher dye...
please include all the information and step by step
procedure.
#2: Gene Translation How many ribosomes are actively synthesizing proteins at any instant in an E. coli cell growing with a doubling time of 45-minutes? Use the following assumptions in your calculations- a. The birth size (size just after mitosis is complete) of E. coli is 1 micron in diameter and 2 microns in length. b. Water content of the cell is 85%. 50% of the dry cell mass is...
Your friend performed gram staining procedure on his bacterial sample, but he forgot to wash his slide with 95% alcohol solution. What result would you expect for his sample and is it reliable? explain B 1 ol Certain diseases such as ALS affects motor neurons (neurons that voluntarily sends impulses) rendering them weak and gradually destroying these neurons. Patients having such diseases suffer from difficulty in walking, speaking, holding up posture, breathing, swallowing, and other symptoms. Explain why they would...
Hy 1.008 664,427 0 15.964 18. A biochemical engineer isolates a bacterial gene fragment and dissolves a 20.6. mg sample of the material in enough water to make 41.5 mL of solution. The osmotic pressure of the solution is 0.340 torr at 25.0°C. (a) What is the molar mass of the gene fragment (5 pts)? 2mci we6 .0206 s .0415 C= .0206 ms.
Most questions answered within 3 hours.
-
Minitab Problem: Take the Lake Hume June rainfall data and find
use the processes outlined in...
asked 2 minutes ago -
X Company is trying to decide whether to continue using old
equipment to make Product A...
asked 3 minutes ago -
IN PYTHON ONLY !! Program 2: Re-work
program #5 (WeeklyHours) from the previous assignment such that...
asked 40 minutes ago -
The average length of time between arrivals at a turnpike
toll-booth is 26 seconds. What is...
asked 2 hours ago -
(a) A piston at 6.1 atm contains a gas that occupies a volume of
3.5 L....
asked 3 hours ago -
Please answer true or false. Words
cannot be changed or added in to make it true...
asked 3 hours ago -
An empty test tube weighs 15.923 grams. Then,
MgCl2•6H2O is added into the test tube. After...
asked 3 hours ago -
Assume memory access is 10 units of time and disk access is
10000 units of time....
asked 3 hours ago -
1. Are all good samples random?
2. Magazines often report surveys giving statistics such as “63%...
asked 4 hours ago -
Under all the various types of market structures, firms
must eventually earn some economic profits for...
asked 3 hours ago -
Consider the following fitness regime for a single locus trait
with two co-dominant alleles: w11 =...
asked 4 hours ago -
A large cable company reports the following.
80% of its customers subscribe to its cable TV...
asked 4 hours ago