Question

1. If a template strand reads: 3’-TTG CAA TGC AAC-5’ what will the new strand read?

2. How are new nucleotide monomers attached to the growing strand? What is the reaction that

takes place? How is dATP different from ATP?

3. Why does the lagging strand exist?

4. In the lagging strand, what is the enzyme that replaces the RNA primer with DNA? What

enzyme then connects the two fragments together?

5. The replication machinery is very accurate but every once in a billion basepairs, a mismatch

occurs. Also, at times DNA can become damaged by environmental factors. Outline the steps

involved in correcting these errors.

6. What is it that exists at the ends of DNA that prevents the erosion of genes during successive

rounds of replication?

7. Describe the details of the above mechanism and how and where telomerase restores the

shortened telomere sequences.

8. When during the cell cycle is DNA replicated? What is the cell getting ready to do if it’s

replicating its DNA?

Answer 1

1. If the template strand sequence is, 3’-TTG CAA TGC AAC-5’ , the new strand or complementary strand sequence is, 5’-AAC GTT ACG TTG-3.’