Agarose gels are created by dissolving powdered agarose into liquid buffer. We will be using a...
Agarose gels are created by dissolving powdered agarose into liquid buffer. We will be using a 0.8% agarose gel (weight in grams/volume in mL). If each gel is made using 100ml of buffer, how many grams of agarose are used? Show your work!
Homework Answers
0.8% basically means 0.8 grams of powder in 100mL of solution.
So for 100mL of buffer, you would need exactly 0.8grams of agarose and add buffer into it until the volume reaches 100mL.
So there should not be any calculation required for this.
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Agarose gels are created by dissolving powdered agarose into
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Can
anyone show me step-by-step on how to do the agarose calculations?
This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
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please hlep me answer those three questions asap.
Please ignore question 7.
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