Homework Answers
Lineweaver -burk plot is
1/V= 1/Vmax + (KMapp/Vmax) 1/S
KM app = KM*(1+I/KI)
Higher substrate concentration overcomes the inhibition effect of I. Hence Vmax remains the same.
Add Answer to:
Consider the following modification of the Michaelis Menten mechanism: E + S ES rightarrow E +...
how we can solve q2 2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant...
how we can solve q2
2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to...
(10 points) Michaelis-Menten kinetics. Consider ving enzymatic reaction, E + A H EA → E+ P...
(10 points) Michaelis-Menten kinetics. Consider ving enzymatic reaction, E + A H EA → E+ P where E, A, and P are the concentrations of enzyme, substrate and product reen Show that rate and product respectively. V = "max [A] Km + [A]
Enzymes are often described as following the two-step mechanism: E+S⇌ES(fast) ES→E+P(slow) Where E = enzyme, S...
Enzymes are often described as following the two-step mechanism: E+S⇌ES(fast) ES→E+P(slow) Where E = enzyme, S = substrate, ES = enzyme-substrate complex and P = product. Question: Molecules that can bind to the active site of an enzyme but are not converted into product are called enzyme inhibitors.Write an additional elementary step to add into the preceding mechanism to account for the reaction of E with I, an inhibitor. Express your answer as a chemical equation.
The Michaelis-Menten equation models the hyperbolic relationship between [S) and the initial reaction rate V, for a...
The Michaelis-Menten equation models the hyperbolic relationship between [S) and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E+S ES E + P. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and [S] >> K. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity V where steady state conditions are assumed. (E l refers to the total enzyme concentration and [Erre refers...
The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate (V0) for...
The Michaelis-Menten equation models the hyperbolic relationship
between [S] and the initial reaction rate (V0) for an enzyme
catalyzed, single substrate reaction: E S ES E P. The model can be
more readily understood when comparing three conditions: [S]Km.
Match each statement with the condition that it describes. Note:
\"Rate\" refers to initial velocity (V0) where steady state
conditions are assumed; [Etotal] refers to the total enzyme
concentration, and [Efree] refers to the concentration of free
enzyme.
categories: [S]<<Km, [S]=Km,...
i need help with all question 5 please! thank you Question 5: Use the data below...
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
PLEASE JUST HELP WITH #5 In addition to competitive inhibition, there are two other common types...
PLEASE JUST HELP WITH #5
In addition to competitive inhibition, there are two other common types of enzyme inhibition. One type is called uncompetitive inhibition. For this mechanism, the inhibitor can only bind to the ES complex and NOT to the free enzyme: 1. kz . E + E+P ES + 1 ESI Follow the following steps (similar, but not identical to problem 2) to derive d[P]/dt for this mechanism. (a) Write the equation for d[P]/dt (b) Write the equation...
The Michaelis-Menten equation models the hyperbolic relationship between (S) and the initial reaction rate V, for...
The Michaelis-Menten equation models the hyperbolic relationship between (S) and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E+ S E S E+P. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and [S] >> K. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity Vwhere steady state conditions are assumed. [Exotal) refers to the total enzyme concentration and [Etre) refers to the...
Enzyme E bind to substrate S to form ES complex leading to product formation. However, the ES com...
Enzyme E bind to substrate S to form ES complex leading to product formation. However, the ES complex also undergoes suicidal inactivation that would result in the loss of the total enzyme activity. In addition, after the binding of S to E there exposes another binding site in E for uncompetitive inhibitor I. The ESI complex does not have the capacity to form product and would not undergo inactivation. The equilibriums, reactions and the kinetic parameters involved can be represented...
The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate V for...
The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate V for an enzyme-catalyzed, single-substrate reaction E+S E S E+P. The model can be more readily understood when comparing three conditions: (S) <<K.. [S] = Km, and [S] >> Km. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity V, where steady state conditions are assumed. Etotal) refers to the total enzyme concentration and Etree refers to the concentration...
how we can solve q2
2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to...
(10 points) Michaelis-Menten kinetics. Consider ving enzymatic reaction, E + A H EA → E+ P where E, A, and P are the concentrations of enzyme, substrate and product reen Show that rate and product respectively. V = "max [A] Km + [A]
The Michaelis-Menten equation models the hyperbolic relationship between [S) and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E+S ES E + P. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and [S] >> K. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity V where steady state conditions are assumed. (E l refers to the total enzyme concentration and [Erre refers...
The Michaelis-Menten equation models the hyperbolic relationship
between [S] and the initial reaction rate (V0) for an enzyme
catalyzed, single substrate reaction: E S ES E P. The model can be
more readily understood when comparing three conditions: [S]Km.
Match each statement with the condition that it describes. Note:
\"Rate\" refers to initial velocity (V0) where steady state
conditions are assumed; [Etotal] refers to the total enzyme
concentration, and [Efree] refers to the concentration of free
enzyme.
categories: [S]<<Km, [S]=Km,...
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
PLEASE JUST HELP WITH #5
In addition to competitive inhibition, there are two other common types of enzyme inhibition. One type is called uncompetitive inhibition. For this mechanism, the inhibitor can only bind to the ES complex and NOT to the free enzyme: 1. kz . E + E+P ES + 1 ESI Follow the following steps (similar, but not identical to problem 2) to derive d[P]/dt for this mechanism. (a) Write the equation for d[P]/dt (b) Write the equation...
The Michaelis-Menten equation models the hyperbolic relationship between (S) and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E+ S E S E+P. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and [S] >> K. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity Vwhere steady state conditions are assumed. [Exotal) refers to the total enzyme concentration and [Etre) refers to the...
Enzyme E bind to substrate S to form ES complex leading to product formation. However, the ES complex also undergoes suicidal inactivation that would result in the loss of the total enzyme activity. In addition, after the binding of S to E there exposes another binding site in E for uncompetitive inhibitor I. The ESI complex does not have the capacity to form product and would not undergo inactivation. The equilibriums, reactions and the kinetic parameters involved can be represented...
The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate V for an enzyme-catalyzed, single-substrate reaction E+S E S E+P. The model can be more readily understood when comparing three conditions: (S) <<K.. [S] = Km, and [S] >> Km. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity V, where steady state conditions are assumed. Etotal) refers to the total enzyme concentration and Etree refers to the concentration...
Most questions answered within 3 hours.
-
A basketball player achieves a hang time, the total time of
flight, of 0.748 s when...
asked 7 minutes ago -
Combustion of an unknown compound containing only carbon and
hydrogen produces 54.9 g of CO₂ and...
asked 6 minutes ago -
A processor of carrots cuts the green top off each carrot,
washes the carrots, and inserts...
asked 19 minutes ago -
Please help me find the coefficient of kinetic and static
friction of two masses (500kg and...
asked 15 minutes ago -
Stanley Department Stores reported net income of $815,000 for
the year ended December 31, 2018.
Additional...
asked 21 minutes ago -
Below are the node_struct, link, list_struct and
list as defined in class and in hw3: typedef...
asked 19 minutes ago -
BA Corp is issuing a 10-year bond with a coupon rate of 6.00
percent. The interest...
asked 27 minutes ago -
How does a cell control the cell cycle? Explain 4 different ways
that cell cycle is...
asked 32 minutes ago -
We have to write some code to simulate
rabbits population growth in Australia. We have determined...
asked 33 minutes ago -
Presidential Aviation has provided charter flights to a wide
array of customers, including business travelers worldwide....
asked 46 minutes ago -
Discuss the significance of accounting systems in generating
reliable accounting information (200 words)
asked 52 minutes ago -
McCracken Aerial,
Inc., produces and sells a unique type of TV antenna. The company
has just...
asked 1 hour ago