Question

cloning Screen a genomic library Screen a cDNA library Southern blot PCR Sequencing (automated)

Sorry the top was on a separate page!

Technique/ Why would you do this? / How do you do this? / What do you need?

Answer 1

cloning: cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually.

Why would you do this:cloning involves creating a cloned embryo for the sole purpose of producing embryonic stem cells with the same DNA as the donor cell. These stem cells can be used in experiments aimed at understanding disease and developing new treatments for diseases.

How do you do this cloning:In reproductive cloning, researchers remove a mature somatic cell, such as a skin cell, from an animal that they wish to copy. They then transfer the DNA of the donor animal's somatic cell into an egg cell, or oocyte, that has had its own DNA-containing nucleus removed.

What do you need:

Human cells,from the individual who will be cloned.

- Media in which these human cells will grow and divide.
(breeding oven)
- Media in which cells will stop dividing, and enter a state
of "quiescence" without dying.(freezer)
- Laboratory supplies: Incubator, Sterile Hood, petri dishes,
microscopes, and tools capable of removing and implanting
cellular organelles,from one cell to another.
- Unfertilized human egg cells.
- Media in which the fertilized eggs can grow and divide.

screen a genomic library:

A genomic library is a collection of the total genomic DNA from a single organism. ... In order to construct agenomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size.