ZuoTi.Pro
Question

The spread plate method requires a series of dilations from an undiluted liquid sample. if you...

The spread plate method requires a series of dilations from an undiluted liquid sample. if you want to spread 0.1 ml of a 1/1000 dilution made from the original sample onto a solidified agar plate, how would you obtain that dilution? (use 10-fold dilitions). You can use a diagram to explain your answer.
science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

We have to do 3 serial of 1ml in 9ml to obtain 1/1000dilution.m Sanyle 00

0 0
Add a comment
Know the answer?
Add Answer to:
The spread plate method requires a series of dilations from an undiluted liquid sample. if you...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...

    You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...

  • A. You have been given a tube of E. coli. You are asked to make 1...

    A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells   +    _____ ml water    = 1 ml    (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...

  • 2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...

    2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....

  • you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of...

    you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of a 1:100 dilution of a 1:50 dilution of a 1:10 dilution of the original sample. What is the concentration of the original sample. What is the concentration of the original culture in cells/ml

  • 9. A. You have diluted a sample by 1000 fold and plated 1 mL on an...

    9. A. You have diluted a sample by 1000 fold and plated 1 mL on an agar plate. You observe 35 colonies. What was the concentration of the original sample in CFU/mL?

  • please help with whatever possible. thank you so much in advance. Name One use of serial...

    please help with whatever possible. thank you so much in advance. Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...

  • b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...

    b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...

  • 7. As part of an ongoing monitoring program, you are testing a sample of water from...

    7. As part of an ongoing monitoring program, you are testing a sample of water from the Chattahoochee River north of Atlanta. Your 10' final dilution (undiluted) plate has 56 Escherichia coli colonies on it. One milliliter was pipetted onto the plate. The EPA safety standards for recreational freshwater state that the water cannot contain more than 235 E. coli per 100 ml. Is the river safe to swim or kayak in? Why or why not?

  • 4. From a culture of M. luteus, you take 10 L and dilute it into 9990uL...

    4. From a culture of M. luteus, you take 10 L and dilute it into 9990uL of sterile water. From this dilution, you take an aliquot of 10uL and plate it onto fresh agar. The following day, you observe 96 colonies on the plate. What is the original CFU/mL of your culture of M. luteus?

  • You are provided below with a data set for three surface spread plates obtained from a...

    You are provided below with a data set for three surface spread plates obtained from a serially diluted (ten-fold) sample of water on EMB agar that have the following types of colonies on them: Number of colonies Dilution plated TYPE A (circular, purple colonies with a metallic green sheen) TYPE B (circular, dull pale pink colonies) -2101 -567 196 18 What is the total viable count for the water sample?

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT