Investigators found that cyclin B concentration rises and falls in synchrony with mitotic events and MPF...
Investigators found that cyclin B concentration rises and falls in synchrony with mitotic events and MPF activity. However, correlation is not causality. How would you show that cyclin B was responsible for these cell cycle events?
Homework Answers
Following procedure was undertaken to prove the above statement:
- Using a frog egg a "cycling" extract was prepared to initiate the early development.
- Using low levels of RNAse mRNA was depleted from this extract. This extract did not cycle.
- Hence, cyclin B mRNA was added to the depleted extract, after RNAse inactivation and cycling was restored.
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Investigators found that cyclin B concentration rises and falls
in synchrony with mitotic events and MPF...
QUESTION 25 The concentration of mitotic cyclin ____________________. A. is highest in G1 phase B. decreases...
QUESTION 25 The concentration of mitotic cyclin ____________________. A. is highest in G1 phase B. decreases at the end of M phase as a result of ubiquitylation and degradation C. decreases at the prophase D. is controlled by phosphorylation QUESTION 26 Which of the following statements about the ubiquitin-proteasome pathway is false? A. Proteases, which reside in the central cylinder of a proteasome, degrade the ubiquitin-modified protein. B. Ubiquitin is a small protein that is non-covalently attached to proteins to...
You are a studying the progression of cells through the cell cycle. You are particularly interested...
You are a studying the progression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcin B/CDK2. Given...
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of...
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcia...
help please!! 21) "The paradigm that the adult heart is a post mitotic organ was established...
help please!!
21) "The paradigm that the adult heart is a post mitotic organ was established in the 1970s. Later, this concept was changed because of the growing body of evidence challenging this traditional view. In adults, myocardium can regenerate from the cardiac stem cells that enter the cell cycle, contributing to changes in the myocardial mass. A low level of cardiomyocyte renewal may occur throughout life, which may be in response to cardiac stem cell activity or migration of...
Hi! I am having trouble answering these questions, more specifically I am stuck on part B! Could you please help me out...
Hi! I am having trouble answering these questions, more
specifically I am stuck on part B! Could you please help me out and
let me know how to answer this?
Thanks!
2) As a continuation of the experiments in Question 1 you want to study the timing of the APC's activity more distinctly. To do this you treat your cells with MG132- a proteasome inhibitor. When MG132 is added to cells proteins can still be ubiquitinated, but they are not...
evens only 1. What is the difference between Change in quantity demanded and Change in demand? 2. True or false? As the price of oranges rises, the demand for oranges falls, ceteris paribus. Expl...
evens only
1. What is the difference between Change in quantity demanded and Change in demand? 2. True or false? As the price of oranges rises, the demand for oranges falls, ceteris paribus. Explain your answer 3. With respect to each of the following changes, identify whether the demand curve will shit rightward or leftward: a An increase in income (The good under consideration is a normal good) b. A nse in the price of a substtute good C. A...
c) How would your answer to (b) above change if consumption in Oregon were a negative...
c) How would your answer to (b) above change if consumption in Oregon were a negative function of the interest rate. Fpr each of the four variables state: rises more, rises less, rises same, falls less, falls more, falls same, no change, or ambiguous. No computations necessary. In 2-3 sentences explain why this assumption for consumer behavior might be more realistic. This exercise is similar to exam question. Please hand it in on Oct 10, 2019. It will be counted...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
2. Match the phase to the description. NOTE: this question might look a little different on...
2. Match the phase to the description. NOTE: this question might look a little different on the Moodle activity. Read carefully. Phase Hanne Description of Events in the Life of a Cell • Anaphase of muitosis A. The combined phases of GI/Go+S+G2 • Gl phase • G2 phase B. The cell is metabolically active; the cell duplicates argumelles and cytosolie components; the cell size starts to increase the cell makes proteins which will soon be used in DNA synthesis C....
10:54 lLTE Notes October 6, 2019 at 10:49 PM 1. How many chromosomes are found in...
10:54 lLTE Notes October 6, 2019 at 10:49 PM 1. How many chromosomes are found in a homologous pair? (.5 pt) 2. How many chromatids are found in a homologous pair? (.5 pt) 3. During what part of the cell cycle are G1 and G2 found? (.5 pt) 4. How many chromosomes are found in a human body cell? (.5 pt) 5. Do all organisms have the same number of chromosomes? (.5 pt) 6. If a cell has 16 pairs...
You are a studying the progression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcin B/CDK2. Given...
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcia...
help please!!
21) "The paradigm that the adult heart is a post mitotic organ was established in the 1970s. Later, this concept was changed because of the growing body of evidence challenging this traditional view. In adults, myocardium can regenerate from the cardiac stem cells that enter the cell cycle, contributing to changes in the myocardial mass. A low level of cardiomyocyte renewal may occur throughout life, which may be in response to cardiac stem cell activity or migration of...
Hi! I am having trouble answering these questions, more
specifically I am stuck on part B! Could you please help me out and
let me know how to answer this?
Thanks!
2) As a continuation of the experiments in Question 1 you want to study the timing of the APC's activity more distinctly. To do this you treat your cells with MG132- a proteasome inhibitor. When MG132 is added to cells proteins can still be ubiquitinated, but they are not...
evens only
1. What is the difference between Change in quantity demanded and Change in demand? 2. True or false? As the price of oranges rises, the demand for oranges falls, ceteris paribus. Explain your answer 3. With respect to each of the following changes, identify whether the demand curve will shit rightward or leftward: a An increase in income (The good under consideration is a normal good) b. A nse in the price of a substtute good C. A...
c) How would your answer to (b) above change if consumption in Oregon were a negative function of the interest rate. Fpr each of the four variables state: rises more, rises less, rises same, falls less, falls more, falls same, no change, or ambiguous. No computations necessary. In 2-3 sentences explain why this assumption for consumer behavior might be more realistic. This exercise is similar to exam question. Please hand it in on Oct 10, 2019. It will be counted...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
2. Match the phase to the description. NOTE: this question might look a little different on the Moodle activity. Read carefully. Phase Hanne Description of Events in the Life of a Cell • Anaphase of muitosis A. The combined phases of GI/Go+S+G2 • Gl phase • G2 phase B. The cell is metabolically active; the cell duplicates argumelles and cytosolie components; the cell size starts to increase the cell makes proteins which will soon be used in DNA synthesis C....
10:54 lLTE Notes October 6, 2019 at 10:49 PM 1. How many chromosomes are found in a homologous pair? (.5 pt) 2. How many chromatids are found in a homologous pair? (.5 pt) 3. During what part of the cell cycle are G1 and G2 found? (.5 pt) 4. How many chromosomes are found in a human body cell? (.5 pt) 5. Do all organisms have the same number of chromosomes? (.5 pt) 6. If a cell has 16 pairs...
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