Question

solution preparation (Freshly prepared by you. Do these calculations before lab.) • Formamide bleach solution: 1.2% H,0,5% formamide in 0.5x SSC. Make it fresh. 50% methanol

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to find the formamide bleach solution (the calculations to prepare it)

Answer 1

Preparation of formamide bleach solution : 1st we have to prepone 0.5X SSC from 200 Ssc. So, we howe to prepene 20x ssc linst. Composition of 20x SSC 3m Nace, 300 mm Sodium citrate 1% Tween-20 in 12 of M :l: - Q. Hoc. Let us make 100me of 208 SSC solution, 3M Nallin 1L H2O means 3X58.449 2 175.329– of Nace in 1 L H₂O. So, to make 100 Hz 20x SSC, 1st we have to add - 17.532g of Nace in toomt 70ml of H₂O. (6) 300 mM Sodium cituate in 100 ml of H2O > Molecular weigh of Sodium Citrate z 258.06 g/mol To prepone 20% SSC (100m) we have to add - 258606x3 10x10 93 7.749 of Sodium Citrate, 258.06 g in 100ome 1 M on 1000 mm 300 mm in 1000 ml 258.06x300 g 300 mm in too il 7 258.06 100g sito enlar weigh imate in 10 1000 00X 1600 1080X36 z 7.744

So, we have to add 7.749 of sodium citrate in e zome of H₂O i Lastly we have to add 1 ml of Tween 20 (to make 1%) in 70 ml of H₂O Lastly we hawe to make the volume up to 100ml by adding H2O Total = 70ml H₂O+ 4 me Tween 20271ml. Pest 629 me So we have preponed Loome 20x SSC, Now, to make 100 ml 0.58 SSC we have to add some 20x SSC into 100 ml H2O. i 20x x V = 0.5X * 100 me ___2) Vz0.5X x 100 me 22.5 ml 20 X So, 1st we have to take 90 ml of H₂O, then we have to add 2.5 ml of 20x Ssc into it & then volume is made up to 100 me by adding rest of H₂O so we have prepare Belone volume makeup, we have to add 1.2me of H2O2 & 5 ml of Sanmamide in 90 ml of H2O Total z gome H2O +2.5ml 20x-sse +1.2 me H₂O₂ + 5ml formamide a 98.7 ml Rest, 100 ml-98.7 ml z 1.3 ml H₂O is added to make 100 ml O.SX sse solution.

Preparation of 50% methanolo Let us make some methand (50%) To make come methanol (50%) we hewe to add 5 ml 100 % methanol in 5 ml H₂O.