Contrast replication of leading and lagging strands in both prokaryotes and eukaryotes.
In prokaryotic cells, there is only one point of origin, replication occurs in two opposing directions at the same time, and takes place in the cell cytoplasm. Eukaryotic cells on the other hand, have multiple points of origin, and use unidirectional replication within the nucleus of the cell. Prokaryotic cells possess one or two types of polymerases, whereas eukaryotes have four or more.
Replication also happens at a much faster rate in prokaryotic cells, than in eukaryotes. Some bacteria take only 40 minutes, while animal cells such as humans may take up to 400 hours. In addition, eukaryotes also have a distinct process for replicating the telomeres at the ends of their chromosomes. With their circular chromosomes, prokaryotes have no ends to synthesize. Lastly, the short replication in prokaryotes occurs almost continuously, but eukaryotic cells only undergo DNA replication during the S-phase of the cell cycle.
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Contrast replication of leading and lagging strands in both prokaryotes and eukaryotes.
In the replication fork, label the leading and lagging strands and the 5' and 3' ends of the DNA and RNA molecules. The 5 and 3' labels are used multiple times.
5a. For the replication fork shown below: - label the leading and lagging strands; - draw arrows to indicate which direction DNA synthesis is proceeding in for each of these strands; - label their 5' and 3' ends. Replication fork movement →→→ 5'-ATCTGGCAGTACGTACTGGATC CGUCAUGC GTCGAATCTGAC-3' CAGCTTAGACTG-5' ATCTGGCTATTCGT 3'-TAGACCGATAAGCATGACCTAG b. Okazaki fragments are generated during (leading / lagging) strand synthesis (circle the correct answer). c. Some U's are included in one of the strands in the figure. Why are there U's...
1a. What is the basis for the difference in how the leading and lagging strands of DNA molecules are synthesized? a. the orgins of replication occur only at the 5' end b. DNA ligase works only in the 3'->5' direction c. helicases are single stranded binding proteins work at the 5' end d. DNA polymerase can join new nucleotides only to the 3' end and of a preexisted strand, and the strand are antiparallel. ------------------------------------------------------------------------------------------------------------------------------------------- 1b. DNA polymerase; a. do...
What figure correctly depicts the direction of replication of the leading and lagging strands at replication fork? (All lines with arrows show the newly synthesized DNA and indicate the direction of polymerization. The 5' and 3 designations are indicated on the template DNA.) A) A B) B C) C D) D E) E
Compare and contrast DNA replication in prokaryotes and eukaryotes. Include three commonalities and three distinctions. Describe the molecular structure of DNA in all organisms. Contrast the differences in DNA structure between prokaryotes and eukaryotes. Compare and contrast DNA and RNA with respect to structure and function. Include three commonalities and three distinctions.
The diagram below shows a replication bubble with synthesis of the leading and lagging strands on both sides of the bubble. The parental DNA is shown in dark blue,the newly synthesized DNA is light blue, and the RNA primers associated with each strand are red. The origin of replication is indicated by the black dots on theparental strands.Rank the primers in the order they were produced. If two primers were produced at the same time, overlap them
c) Compare and contrast DNA replication in prokaryotes and eukaryotes. Your answer should include a description of replication as it would occur in these two types of cells.(20)
A single replisome complex can simultaneously synthesize both the leading and lagging strands of DNA within a moving replication fork. (a) Why is this challenging? (b) Use the “trombone model” analogy to explain how a single replisome synthesizes the lagging strand at the same time the leading strand is being made.
What element of replication is used in both prokaryotes and eukaryotes? topoisomerase chromatin assembly factors replication licensing factors shelterin telomerase
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the formation of Okazaki fragments in the leading strand. (C) is a consequence of replicating both strands of template DNA at a single replication fork. (D) requires its own replisome.