Question

aganism it to fam 4 Stable bens with other elements of crganism beght ein the enuknnent (carten cycle) Bekd Bor in a dynamic steady state? nyof Concentattin PemMalu Reagent Preparation and Cell Biology Calculations 1. To start you off a problem from Biology 3: Make 500 ml of the following solution: 10 mM Tris-HCI; pH=7.5; 20 mM Glucose; 2% Tween-20; 0.05% BSA Molecular weight of Tris-HCl = 157.6 g/mol. You have a 1M glucose stock. Use it to make the solution. Tween-20 is a liquid. BSA is a solid. %3D Gow)=5000el 157.6 Smely use? you How much of Tris-HCl do 0.011rlomL use? How much of the 1M glucose stock do use? you How much Tween-20 do you (0.5)(0.0)=6. How much BSA do you use? 1. Show all your calculations. 2. Briefly describe the process of making the solution. ocUSMel y I57.6g let's make the problems more realistic...There are multiple correct ways to approach problems in the la e an approach that is direct, conserves materials and is practical in the lab. Consider that you need to maintain reasonable volumes. igh out locs than 1 mg

Answer 1

) Tris HCl neede d to make 50om of Trin- HCl solution Lomahal 157g mor Losamanol ing V, 4 = Vzl2 ahere, v, = Initial volume of stork soluti %23 Imiti'al ioneenr abien of stork solution V= Fimal volume of the selution G= Finel conceuv ati'on of solution, here, VI = ?, e= IM, V2 = 500 me ノ = 0.02 M 20 X10-3 M G = 20 mm %1 : Jom of 1 M glucose stock solution needed.

# 2:%- means Tween -20 Tween-2o solu tion needs 2 mi I0 500 2. Tweew-Lo = 10 m 10 M tueen - 20 needed. 0.05% BSA solubion mean 23 B SA 100 solution needs o.05 SD.0 500 u 0.25 BSA 0 25 BSA s honld be vsed. the solution of desired volume 4 desived con eeutr atiom To make 500ml beaken we need add in a graduated guecose salm Trin-H4 + 10ml IM + 10 mal tween -20 + 0.25 ator iegingramall porti an y of Nater PH ohould be adj us lid base Then volume of the total solutin hen to be made ky adding distilled wuter upto the somark ot keeaker