Question

You have the following items ready to use for making solutions: Glucose (solid; Mw=180g/mol) 1M Tris HCl (liquid solution;MW- 158g/mol) Acetone (liquid; MW-589/mol) 10% agarose (liquid solution: Mw-631g/mol) Your instructions state that you need to make 800ml of 70% acetone. Which equation should you use to determine how much of your acetone source you need? O M= moles/L 0 % = solute amount (g or mis)/total volume x 100
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Answer #1

% = (amount of solute (g or mls) / total volume) * 100

70 = (x / 800 ml) *100

x = (70/100)*800 ml

x = 560 ml

If 560 ml of acetone diluted into total volume of 800 ml the resulting solution would be 70% of acetone

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