Thank you to whoever can help me on this.
Shown below are results from an
immunoprecipitation/immunoblotting experiment. Cell lysates were
immuno-precipitated with ErbB-2 or a control (mIgG) antibody and
immunoblotted with antibodies to importin β1 or to ErbB-2 as shown
in Figure.
ErbB-2: a receptor found on the plasma membrane of mammalian cells
have been shown to move from the plasma membrane to the
nucleus.
Various RNA interference (SiRNA treatments) :
-: no SiRNA; Imp: importin specific SIRNA and N.S.: Non-specific
SiRNA
wild-type (WT) ErbB-2
mutant ErbB-2 lacks nuclear localization signal (ΔNLS)
Shown below are results from an immunoprecipitation/immunoblotting
experiment.
Answer the following questions:
What is the objective of experiment referring to Fig a?
What can you conclude from top panel of figure a where importin
antibody is being used?
What can you conclude from bottom panel of figure a where ErbB2
antibody is being used?
What is the objective of experiment referring to Fig b?
What can you conclude from figure b?
A- ErbB-2 localization depends on the important B-1
B- In the top panel, it is shown that upon using the siRNA against the importin B-1 its expression level in drastically decrease which can be seen in the left panel in the cytoplasmic fragment. Since its expression drastically decreases the level of importin B-1 is nucleus has almost become negligible.
C- As we can see that upon using the siRNA of importin B-1, the levels of ErbB2 is not decreased in the cytoplasm but it was decreased in the nuclear fragment.
D- The main objective of figure B was to check if importin B-1 plays any role in the localization of ErbB2 to the nucleus.
E- It was evident from the figure B that ErbB2 binds to the importin via its NLS. When NLS was deleted ErbB2 was failed to localize in the nucleus
Thank you to whoever can help me on this. Shown below are results from an immunoprecipitation/immunoblotting...
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