Question

can someone help me write an introduction for this lab? thank you. this is how to do it for the first pic, 2nd pic is the actual lab.

Introduction Biochemistry Lab Reports Laboratory reports must be received by 5:00pm on the due date unless previous Late reports will be docked 10% per arrangements have been made with Dr Marzena day. The laboratory reports should follow the format described below Biochemistry Laboratory Report Outline Expectations -Please write the report in your own words Introduction/Objective of the Lab: Write an introductory paragraph Materials and Methods: describing the background and rationale for the lab uo Give a brief outline of the procedure (overview or outline)う oroutine)→ fi-Pures. musrbe Results/Data Description ofdata andor calulations→ Description of data and/or calculationsxPlanuhat 2,odagenue enamfun Summary of your results Discussion: Interpret your results. (Answer questions of this type: "What did you learn?". Was the experiment successful?", "Any problems?"·"Did you accomplish the experimental objective?") objectve) di scuss vheresuutindetai Questions: Answer any included questions either separately, or as part of the discussion. If included ndhe part f the dasin included with the discussion, please underline the answers. Note: Submit raw data, properly annotated, with report

Answer 1

Introduction to enzyme kinetics: -

Enzyme kinetics is always studied taking any one enzyme as base. In the given experiment, we are taking the enzyme phosphatase as base. Phosphate reacts with colorless p-nitrophenol phosphate (NPP) substrate, to give yellow p-nitrophenol as product. Now, if we have to find out the optimum enzyme concentration and optimum substrate concentration, then we will have to vary the enzyme and substrate concentration in two different experiments.

In the experiment where we are finding the optimum substrate concentration; we will take 8 test tubes with varying concentration of substrate, but with the same concentration of enzyme. As blank we will keep the enzyme solution, but will not add the substrate.

On the other hand, in the experiment where we are finding the optimum enzyme concentration; we will take 8 test tubes with varying concentration of enzyme, but with the same concentration of substrate. In the test tube taken as blank, we will add the substrate but will not add the enzyme.

In all cases, O.D. will be noted down using UV-Vis spectrophotometer at 575nm.