If a soluble protein with a KDEL sequence at its C-terminus is misfolded in the ER...
If a soluble protein with a KDEL sequence at its C-terminus is misfolded in the ER lumen, it will ________.
a. be transported to the Golgi apparatus and beyond without any delay
b. stay in ER after being correctly refolded
c. be transported to the Golgi apparatus and beyond after being correctly refolded
d. None of the above.
Homework Answers
If a protein having KDEL sequence ,it shows that it is ER resident protein. In Er chaperone are present that help in correct folding of misfolded protein. So folding of protein occur in the Er lumen .so protein remain in ER after being correctly refolded .
Option B is correct statement...
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If a soluble protein with a KDEL sequence at its C-terminus is
misfolded in the ER...
What would you expect to happen to a newly synthesized soluble protein if a mutation caused...
What would you expect to happen to a newly synthesized soluble protein if a mutation caused the deletion of the KDEL sequence? a. The protein would be returned to the ER in COPI-coated vesicles. b. The protein will be carried forward through the Golgi apparatus. c. The protein will be trapped in COPII vesicles . d. The protein will be incorporated into COPI vesicles. e. The protein will be recognized by KDEL receptors and a retrieval sequence (KDEL sequence) will...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
need help with 4i, ii, and iii please. 4) A cell line is transfected with a...
need help with 4i, ii, and iii please.
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or a variant of X that contains the amino acids KDEL at its C terminus, both of them under control of an inducible promoter. KDEL motifs are normally found on proteins located in the lumen of the ER. At 30 minutes after induction, cycloheximide, an inhibitor of protein synthesis, is added to the culture medium. At 30,...
Drag the terms on the left to the appropriate blanks on the right to complete the...
Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all. Reset Help transport vesicle(s) Soon after initiation of translation of an mRNA coding for a secretory protein, the of the secretory protein is synthesized and is exposed on the surface of the ribosome. The signal recognition particle (SRP) contains the that marks this protein for into the endoplasmic reticulum cotranslational translocation...
Order the following statements to correctly describe the route of secreted protein as it is synthesized...
Order the following statements to correctly describe the route of secreted protein as it is synthesized and secreted from a eukaryotic cell. A. The protein folds and undergoes modification within the rough ER. B. The protein travels within a membranous vesicle from the ER to the cis face of the Golgi apparatus. C. Translation begins on a ribosome. D. The protein travels toward the trans face of the Golgi, from which it is packaged into a secretory vesicle. E. The...
Which protein modification does NOT take place in the ER lumen? A. Polyubiquitylation &nbs
Which protein modification does NOT take place in the ER lumen? A. Polyubiquitylation B. N-linked glycosylation C. Cysteine bridge formation D. chaperone-mediated folding E. GPI anchoring 2. Which of these Eukaryotic proteins is least likely to exhibit an ER import sequence during translation? A. Glutathione B. CFTR C. Protein disulfide isomerase D. Acetylcholine receptor E. Na+-K+-ATPase 3. Which function is NOT attributed correctly to either smooth ER (SER) or rough ER (RER)?...
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or...
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or a variant of X that contains the amino acids KDEL at its C terminus, both of them under control of an inducible promoter, KDEL motifs are normally found on proteins located in the lumen of the ER. At 30 minutes after induction, cycloheximide, an inhibitor of protein synthesis, is added to the culture medium. At 30, 45, and 60 minutes after induction (that is,...
please answer both Which of the following is NOT TRUE regarding both M6P and KDEL receptors?...
please answer both
Which of the following is NOT TRUE regarding both M6P and KDEL receptors? Select one: a. They help transport cargo to their proper destinations b. They bind to cargo proteins in the Golgi apparatus. c. They are transported by vesicle budding and fusion, d. They move back-and-forth between different organelles. O e. They release soluble cargo proteins at lower pH. Which of these is NOT a function of the Signal Recognition Particle (SRP) involved in transport of...
Help please. Thanks 14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic...
Help please. Thanks
14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic AMP d) AMP 15. When adrenalin is produced PKA is turned off a) b) PKA is turned on c) Has no effect on PKA d) None of the above 16. All proteins with a signal sequence enter the ER 17. The sorting signal, Nuclear Localization Sequence, all proteins in the cytosol to enter the 18. In order for proteins to enter the mitochondria, they...
Which of the following statements describing the import of proteins into the nucleus is correct? Answers...
Which of the following statements describing the import of proteins into the nucleus is correct? Answers A-D A Transport into the nucleus requires the activity of a G protein. B The receptor for nuclear proteins is found in the cytosol and travels into the nucleus with the protein being transported o С Proteins destined for the nucleus are transported in a fully-folded conformation. D These statements are all correct. o You are interested in a water-soluble protein that functions within...
need help with 4i, ii, and iii please.
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or a variant of X that contains the amino acids KDEL at its C terminus, both of them under control of an inducible promoter. KDEL motifs are normally found on proteins located in the lumen of the ER. At 30 minutes after induction, cycloheximide, an inhibitor of protein synthesis, is added to the culture medium. At 30,...
Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all. Reset Help transport vesicle(s) Soon after initiation of translation of an mRNA coding for a secretory protein, the of the secretory protein is synthesized and is exposed on the surface of the ribosome. The signal recognition particle (SRP) contains the that marks this protein for into the endoplasmic reticulum cotranslational translocation...
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or a variant of X that contains the amino acids KDEL at its C terminus, both of them under control of an inducible promoter, KDEL motifs are normally found on proteins located in the lumen of the ER. At 30 minutes after induction, cycloheximide, an inhibitor of protein synthesis, is added to the culture medium. At 30, 45, and 60 minutes after induction (that is,...
please answer both
Which of the following is NOT TRUE regarding both M6P and KDEL receptors? Select one: a. They help transport cargo to their proper destinations b. They bind to cargo proteins in the Golgi apparatus. c. They are transported by vesicle budding and fusion, d. They move back-and-forth between different organelles. O e. They release soluble cargo proteins at lower pH. Which of these is NOT a function of the Signal Recognition Particle (SRP) involved in transport of...
Help please. Thanks
14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic AMP d) AMP 15. When adrenalin is produced PKA is turned off a) b) PKA is turned on c) Has no effect on PKA d) None of the above 16. All proteins with a signal sequence enter the ER 17. The sorting signal, Nuclear Localization Sequence, all proteins in the cytosol to enter the 18. In order for proteins to enter the mitochondria, they...
Which of the following statements describing the import of proteins into the nucleus is correct? Answers A-D A Transport into the nucleus requires the activity of a G protein. B The receptor for nuclear proteins is found in the cytosol and travels into the nucleus with the protein being transported o С Proteins destined for the nucleus are transported in a fully-folded conformation. D These statements are all correct. o You are interested in a water-soluble protein that functions within...
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