Please give correct answer and an explanation why. Thank you.
Charge of the protein molecules
Gel electrophoresis is used to separatemacromolecules like DNA, RNA and proteins.
DNA fragments are separated according to their size.
Proteins can be separated according to their size and their charge (different proteins have different charges)
Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.
Proteins are separated by charge in agarose because the pores of the gel are too large to sieveproteins.
In agarose gel electrophoresis, proteins with a strong negative charge move fastest towards the positive side of the gel, whereas positively charged proteins move in the opposite direction.
This technique might be used to separate proteins that have the same molecular weight but different charges, or when size is not important.
Please give correct answer and an explanation why. Thank you. The migration of a protein in...
can you please answer all three questions. Thank you so much!! QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
Please give an explanation as to why it is the correct answer, thank you. Use the following data to answer questions 13 - 16 €° (volts) Mn2+ + 2 e → Mn -1.03 Fe3+ + 3 e → Fe 9 -0.04 oglo biso 13. Which of the following statements is (are) true? a) The SHE would act as the cathode in a reaction with a Mn anode. b) Mn2+ will react spontaneously with Fe. c) Mn will reduce Fe to...
Based on the migration of 3 DNA fragments {A, B, & C) of the indicated sizes (where kb = kilobases, ie thousand base pairs) in these gels, which of the following statements is correct? А В С А В С kb 0.5 Gel #1 Gel #2 Select one: a. Gell #1 has a higher % agarose than gel #2 b. An 8% acrylamide gel would give greater resolution of fragments larger than 500 bp than either of these gels. c....
Please TYPE your answer and please give the correct answer. Thank you! If the time order of production has not been recorded in a set of data from a process, is it possible to detect the presence of assignable causes? Why or why not?
The answer below is incorrect, please explain why it’s incorrect and give the correct answer. Thank you. 3- Go) iven irei 12 412 /e) Vots .3 2 3ec .5
Please give a brief explanation as to why the following answers are correct. a. Which solution should be the strongest electrolyte: 0.20 M BaCl2, 0.25 M NaCl, or 0.50 M sucrose? Answer: 0.20 M BaCl2 > 0.25 M NaCl > 0.50 M sucrose. Give an explanation as to why this answer is correct involving the number and kinds of particles present in solution. Explanation: B. Arrange the substances water (H2O), propane (C3H8), and ethanol (CH3CH2OH) in terms of their predicted...
Please Help with simple explanation .. please answer at least the last 3 questions if can't estimate ,, please answer the first Q only 3654 Biochemistry Expt. 7 (cont.) Name with reducing agents A B A B (5pts) Protein A contains 2 polypeptide chains (1 of 70,000, 1 of 35,000) held together by disulfide bonds. Protein B contains 2 polypeptide chains (each -75,000) held together by hydrophobic interactions and electrostatic forces. On the SDS-PAGE gel to the right, sketch the...
Explanation of SDS-PAGE for Western blotting procedure, PLEASE answer the questions BELOW: 1. What does a gel electrophoresis allow you to do? 2. What is a gel? 3. How do you make the protein move, and why does this work? 4. Which protein fragments travel the furthest and why? 5. Name 3 materials used to make a gel. 6. What is polyacrylamide? 7. What is the purpose of the buffer? 8. What is the comb (in the gel) for? 9....
Can someone give a thorough explanation of why? Thank you so much!! 4. If V is known at only a single point in space, can E be found at that point? Explain your answer.
please give a brief explanation to why you chose the answer 11. If A is a 3 × 5 matrix, then the transpose of A is O A. A number (possibly non-zero). O B. Zero. OC. A 3 x 5 matrix. O D. A subspace of R3 ○ E. Undefined F. A subspace of R5. O G. A 5 x 3 matrix.