Question

Fill in the following chart with important factors and sequences required for pol I transcription. Then...

Fill in the following chart with important factors and sequences required for pol I transcription. Then complete the chart as if there was a mutation in that factor or sequence.

Factor or Sequence

Describe the consequences

Universal or gene specific

What would the RNA look like?

Why?

UCE

UBF1 unable to bind

Gene specific

Same but lower levels

Only basal transcription would occur

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Answer #1
TIF-IB DIfficulty for SL1 in binding to the sequence Universal lower level Only basal transcription would occur
HMG-boxes The UCE and the core elements do not come into contact. Universal lower levels basal transcription
SL1 SL1 is unable to bind to the promoter gene specific lower levels basal transcription
TTF-I Unable to terminate correctly universal higher levels Transcription continues as termination is hindered
RRN3 Unable to initiate specifically gene specific lower levels basal transcription
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