Question

1. What is the role of the transcription factors? A mutation exists in transcription factors that causes them to bind slightly downstream of the TATA box, causing them to cover the first 3 nucleotides of a gene. RNA polymerase can still transcribe the gene to mRNA, but it misses the first 3 nucleotides. How would this impact translation?
2. RNA is single stranded, and as such, undergoes rapid rates of mutation. How would this affect the ability of siRNAs to combat RNA viruses?
3. Why does translation occur in a 5’ to 3’ direction when DNA is read in a 3’ to 5’ direction?

Answer 1

Transcription factors are actually proteins that are involved in the conversion of DNA to RNA.TATA box is a DNA sequence that indicates,where a generic seq can be read or decoded.if a mutation exist in transcription factors which causes them to bind slightly downstream the outcome will be another molecule of RNA and the resultant protein molecule formed will change.

Translation occurs in 5' to 3' direction because the role of mRNA reading frame is translated from 5'-3' direction.,,by aminoacids into ribosomes by a polypeptide.