inA rapid and reliable method for the separation of lipid classes is described using aminopropyl disposable columns. This method is a modification to an existing procedure that allows the separation of both neutral and acidic phospholipid fractions and a high recovery of the latter.
Acidic phospholipids were eluted with a mixture of hexane-2-propanol-ethanol-0.1 M ammonium acetate-formic acid 420:350:100:50:0.5 containing 5% phosphoric acid after neutral phospholipids had been eluted with methanol.
It was verified that extremely high recoveries of cholesterol (CH), triglycerides (TG), free fatty acids (FFA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidic acid (PA), sphingomyelin (SM), and cerebrosides were obtained with this method.
In addition, there appeared to be no preferential losses or degradation of any particular molecular species as the fatty acid distribution of bovine brain PS and the molecular species profile of plant PI were unaltered by the procedure. Depending on the tissue, this method may yield fractions containing pure lipid classes and/or simple mixtures of lipid classes of similar polarity.
()2. I discovered a new mushroom species, Agaricus flurkeatus, and identified some of the lipids in...
32. Which of the following lipids contain a carbohydrate as part of it's structure: A. Cerebroside B. Ganglioside C. Ceramide D. All of the above E. A and B only 33. Which of the following is a ketone body? A. Succinate B. B-hydroxyacetate C. Acetoacetate D. Acetyl CoA E. Alpha-Ketoglutarate 35. Which of the following is true about the regulation of fatty acid oxidation? A. Acetyl CoA inhibits thiolase B. Increased consumption of glucose increases fatty acid oxidation C. Carnitine...
identify your answer. (2 pts ea) 12) Which statement is NOT true? A) There are many different types of lipids. B) Lipids are found in cell membranes. C) Some hormones are lipids D) Lipids are soluble in organic solvents. E) All lipids contain fatty acids. 13) The process of building up new molecules in the coll is called A) glycolysis. B) catabolism. C) anabolism. D) metabolism E) transamination. 14) Which of the following does not require energy from ATP hydrolysis?...