I want to make 6 samples (each sample 0.5 mL) of BSA ranging from ~100-2000 micrograms/...
I want to make 6 samples (each sample 0.5 mL) of BSA ranging from ~100-2000 micrograms/ mL from a stock solution that is 10.0 milligrams/ mL. Show 6 calculated samples and all steps.
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I want to make 6 samples (each sample 0.5 mL) of BSA ranging
from ~100-2000 micrograms/...
From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions...
From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume...
help Describe in detail how you would make a series of four standards ranging from 1...
help
Describe in detail how you would make a series of four standards ranging from 1 to 100 ppm given the following constraints. a. Your stock solution is 1000 ppm. b. The available pipets have volumes of 2.00 mL, 3.00 mL, and 5.00 mL. c. The available volumetric flasks have volumes of 10.00 mL, 25.00 mL, 50.00 mL, and 100.00 mL. d. You need to make at least 10.00 mL of each of your four standards.
please show work so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml...
please show work
so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
You want to prepare 50 mL of a 20% NaCl salt solution from a 100% stock....
You want to prepare 50 mL of a 20% NaCl salt solution from a 100% stock. Calculate the amount of stock as well as the amount of water you need to use to make this dilution. Assume that you have unlimited volume of the stock.
You want to make 500 mL of 10 M NaOH solutions? How much NaOH do you...
You want to make 500 mL of 10 M NaOH solutions? How much NaOH do you have to weigh out? Walk me through the steps it takes to make this solution? During the weighing out process you weigh out 201.53 NaOH, what is your new molarity? Using your stock solution, you want to make a series of dilutions, you want 25 mL of the following concentrations of NaOH: 0.5 M ,1 M, 3 M, 7 M, 9 M. How much...
Need major help 1. How many milligrams of Ruthenium-tris(2,2'-bipyridyl) dichloride [Ru(bpy)3] do you need to make...
Need major help
1. How many milligrams of Ruthenium-tris(2,2'-bipyridyl) dichloride [Ru(bpy)3] do you need to make one hundred microliters of a 100 mM solution?: 2+ The molecular weight of Ru(bpy)3 is 640.53 grams/mole. N. 2. How many microliters of the 100 mM [Ru(bpy)] solution do you need to make one milliliter of a 10 μM solution? 3. How many samples can we make from this stock solution, assuming one mL each of a 10 uM sample?
Lab Homework Convert 875 ul to ml and to I. Each answer should use scientific notation....
Lab Homework Convert 875 ul to ml and to I. Each answer should use scientific notation. Show your work. Calculate how you will make the following solutions. You will need to show how much of Y solution you will need and how much water you will need. Show your work: You need to make 125 ml of a 0.3 M sodium acetate (NaAc) solution. Your stock solution is 3 M NaAc. You need to make 750 mL of a 1X...
Please help me solve these problems. I don't understand 1. Placing a 0.5 ml sample of...
Please help me solve these
problems. I don't understand
1. Placing a 0.5 ml sample of bacteria into a 4.5 ml sterile water, then transferring a 0.5 ml sample from that dilution to another 4.5 ml sterile water produce a dilution. 2. A technologist in a hospital lab who is trying to determine the concentration of Staphylococcus aureus in a urine sample completed a total plate count. After plating 0.1mL in duplicate, and incubating the plates at 37°C for 24...
calculate the theoretical ph of solution F and N. MLIM HNO, solution 260 ml. De water...
calculate the theoretical ph of solution F and N.
MLIM HNO, solution 260 ml. De water 30 mL 0.1 M NaOH solution PROCEDURE The pH meter should already be calibrated at pH 4, 7 and 10. If your pH meter appears not to be operating properly, notify your TA and they will recalibrate the meter. Please handle the pH probe carefully the tip is made of a fragile glass membrane. Be sure to rinse the probe with water and blet...
help
Describe in detail how you would make a series of four standards ranging from 1 to 100 ppm given the following constraints. a. Your stock solution is 1000 ppm. b. The available pipets have volumes of 2.00 mL, 3.00 mL, and 5.00 mL. c. The available volumetric flasks have volumes of 10.00 mL, 25.00 mL, 50.00 mL, and 100.00 mL. d. You need to make at least 10.00 mL of each of your four standards.
please show work
so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
You want to make 500 mL of 10 M NaOH solutions? How much NaOH do you have to weigh out? Walk me through the steps it takes to make this solution? During the weighing out process you weigh out 201.53 NaOH, what is your new molarity? Using your stock solution, you want to make a series of dilutions, you want 25 mL of the following concentrations of NaOH: 0.5 M ,1 M, 3 M, 7 M, 9 M. How much...
Need major help
1. How many milligrams of Ruthenium-tris(2,2'-bipyridyl) dichloride [Ru(bpy)3] do you need to make one hundred microliters of a 100 mM solution?: 2+ The molecular weight of Ru(bpy)3 is 640.53 grams/mole. N. 2. How many microliters of the 100 mM [Ru(bpy)] solution do you need to make one milliliter of a 10 μM solution? 3. How many samples can we make from this stock solution, assuming one mL each of a 10 uM sample?
Lab Homework Convert 875 ul to ml and to I. Each answer should use scientific notation. Show your work. Calculate how you will make the following solutions. You will need to show how much of Y solution you will need and how much water you will need. Show your work: You need to make 125 ml of a 0.3 M sodium acetate (NaAc) solution. Your stock solution is 3 M NaAc. You need to make 750 mL of a 1X...
Please help me solve these
problems. I don't understand
1. Placing a 0.5 ml sample of bacteria into a 4.5 ml sterile water, then transferring a 0.5 ml sample from that dilution to another 4.5 ml sterile water produce a dilution. 2. A technologist in a hospital lab who is trying to determine the concentration of Staphylococcus aureus in a urine sample completed a total plate count. After plating 0.1mL in duplicate, and incubating the plates at 37°C for 24...
calculate the theoretical ph of solution F and N.
MLIM HNO, solution 260 ml. De water 30 mL 0.1 M NaOH solution PROCEDURE The pH meter should already be calibrated at pH 4, 7 and 10. If your pH meter appears not to be operating properly, notify your TA and they will recalibrate the meter. Please handle the pH probe carefully the tip is made of a fragile glass membrane. Be sure to rinse the probe with water and blet...
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