Homework Answers
Map distance = recombinant / total no of offspring x 100
= 148+ 152/ 1000000
= 300/1000000
=.0003x100
= 0.3cM
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3. *A transformation experiment was carried out with a tvrtade+ donor and a tyr- ade- recipient....
A transformation experiment is performed with a donor strain of M. falcieri that is resistant to...
A transformation experiment is performed with a donor strain of M. falcieri that is resistant to four drugs: A, B, C, and D. The recipient is sensitive to all four drugs. The transformed recipient cells are split in aliquots and plated on media containing various combinations of the drugs. The results are below. All drugs are added at the same time. Drug(s) added # of colonies Drug(s) added # of colonies None 10,000 BC 50 A 1156 BD 48 B...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
5. Using Pl as a generalised transducing phage grown on a cys' pur trp" bacterial donor,...
5. Using Pl as a generalised transducing phage grown on a cys' pur trp" bacterial donor, a recipient strain of genotype cys pur* trp was infected and incubated. In experiment Icys transductants were selected and in experiment II trp* transductants wer,selected. (Note: cys, trp and pur genes are involved in cysteine, tryptophan and purine biosynthesis respectively) (a) What media were used for these selection experiments? Next, the transductants were examined for the presence of the unselected donor markers (genes), with...
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20...
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Genetics Question: I am confused on the second part of the question- determining the information for...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
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III. You isolate a new species of bacteria that only grows in the Antarctic. A wild...
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(A)--Five different experiments are carried out. In each experiment, a block is attached to a horizontal...
(A)--Five different experiments are carried out. In each experiment, a block is attached to a horizontal spring. The block is pulled back a certain distance and released. The block oscillates back and forth on a frictionless surface. Rank the maximum force on the block for each of the following situations. (Rank the smallest force as 1). 1 2 3 4 5 A block of mass M is attached to a spring with a spring constant 2k, pulled back a distance d,...
BG35.2 Enthalpy of Formation of Mgo Page 9 of 12 PRELAB QUESTIONS 1. Read the writeup through the Data Analysis section. A student carried out the experiment described there. The student used 0...
BG35.2 Enthalpy of Formation of Mgo Page 9 of 12 PRELAB QUESTIONS 1. Read the writeup through the Data Analysis section. A student carried out the experiment described there. The student used 0.4894 g of Mg in the first reaction and found that the temperature of the solution went from 22.1 to 42.7'C. In the second reaction, 0.803 g of MgO was used and the solution went from 22.7 to 28.7°C. Calculate the following (show the calculations beside and/or below...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM 5cM 40cM If a diploid female heterozygous for A and D (A D//a d) is testcrossed to a homozygous recessive male (a d/a d), what percent of the progeny are predicted to have the genotype A d/a d? A. 100 B. 75 C. 50 D. 25 E. Can’t be determined 2. Yellow body (y), vermillion eye color (v) and miniature body (m) are recessive...
This experiment was based on Aldol-dehydration reaction using unknown aldehydes and ketones. We carried out an...
This experiment was based on Aldol-dehydration reaction using
unknown aldehydes and ketones. We carried out an aldol condensation
between an unknown aldehyde and an unknown ketone. Here are the
list of possible unknown aldehydes and ketones that were used.
Here is the NMR of the final product. Please predict the
structure given the melting point is 163 degrees Celsius.
The analysis of the 1H NMR spectrum should include a detailed
assignment of each of the NMR resonances to the protons...
5. Using Pl as a generalised transducing phage grown on a cys' pur trp" bacterial donor, a recipient strain of genotype cys pur* trp was infected and incubated. In experiment Icys transductants were selected and in experiment II trp* transductants wer,selected. (Note: cys, trp and pur genes are involved in cysteine, tryptophan and purine biosynthesis respectively) (a) What media were used for these selection experiments? Next, the transductants were examined for the presence of the unselected donor markers (genes), with...
QUESTION 1 Imagine you are preparing an industrial enzyme assay. The protocol says to add 20 mL of 100 mM NaCl to your assay, and the final volume of the assay is 100 ml. What is the working concentration of NaCl? The number of significant digits you include in your answer doesn't matter, but it must be in units of mM. QUESTION 2 Imagine you are preparing an enzyme assay that requires a working concentration of 10 mM NaCl. If...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
BG35.2 Enthalpy of Formation of Mgo Page 9 of 12 PRELAB QUESTIONS 1. Read the writeup through the Data Analysis section. A student carried out the experiment described there. The student used 0.4894 g of Mg in the first reaction and found that the temperature of the solution went from 22.1 to 42.7'C. In the second reaction, 0.803 g of MgO was used and the solution went from 22.7 to 28.7°C. Calculate the following (show the calculations beside and/or below...
This experiment was based on Aldol-dehydration reaction using
unknown aldehydes and ketones. We carried out an aldol condensation
between an unknown aldehyde and an unknown ketone. Here are the
list of possible unknown aldehydes and ketones that were used.
Here is the NMR of the final product. Please predict the
structure given the melting point is 163 degrees Celsius.
The analysis of the 1H NMR spectrum should include a detailed
assignment of each of the NMR resonances to the protons...
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