Predict GAL1 transcription level in glucose and in galactose and explain why a. Deletion of TATA...
Predict GAL1 transcription level in glucose and in galactose and explain why
a. Deletion of TATA box in the promoter of GAL1
b. Deletion of both Gal80 and Gal4 from the genome
c. Deletion of Gal80 and Mig1 from the genome
Homework Answers
a. Deletion of TATA box in the promoter of GAL1 will inhibit the transciption level of GAL1 as the RNA polymerase will fail to bind to the promoter and initiate transcription.
b.Deletion of Gal 80 and Gal4 from the genome will inhibit the transcription level of GAL1 as binding of Gal 4 protein to Upstream activating complex i.e UASG is necessary for initiation of transcription from GAL1.In absence of glucose,Gal80 protein binds to Gal4 protein to inhibit the transcription inititation of Gal 80 but as Gal80 is deleted from genome there would be no transcription.
c.Since Gal80 and Mig 1 inhibit transcription from GAL1,eletion of Gal80 and Mig1 from the genome would not ainhibit the transcription and transcription will continue.
Add Answer to:
Predict GAL1 transcription level in glucose and in galactose and
explain why
a. Deletion of TATA...
Answer all questions and explain in detail. 1) GAL80 loss-of-function mutants express GAL1, 2, 7, 10,...
Answer all questions and explain in detail. 1) GAL80 loss-of-function mutants express GAL1, 2, 7, 10, genes constitutively (in the absence of galactose) because a transcriptional activation by GAL4 is not inhibited b GAL80 is not present to block GAL4 DNA-binding c GAL80 cannot respond to GAL3 signaling d A and B e B and C 2) Gal80-; GAL4- double mutants express the GAL1, 7, 10 genes a only In the presence of galactose b only in the absence of...
GAL80 loss-of-function mutants express the GAL1, 2, 7, 10 genes constitutively in the absence of galactose)...
GAL80 loss-of-function mutants express the GAL1, 2, 7, 10 genes constitutively in the absence of galactose) because A. transcriptional activation by GAL4 is not inhibited B. GAL80 is not present to block GAL4 DNA-binding C. GAL80 cannot respond to GAL3 signaling D. A and B E. B and C TB 68
Please explain your answers as best as you can. I want to be able to understand....
Please explain your answers as best as you can. I want to be
able to understand.
The first drawing below represents the transcription start site and upstream region of a eukaryotic gene. In addition, four deletion constructs are shown, where the unboxed area represents a deletion of upstream DNA. Note the location of the +1 start site and the promoter, which includes the TATA box Non-deleted DNA template and DNA of the various deletions constructs were added to both systems...
Classify each statement according to whether it applies to general transcription factors only, specific transcription factors...
Classify each statement according to whether it applies to general transcription factors only, specific transcription factors only, both, or neither These transcription factors are found in both prokaryotes and eukaryotes. These transcription factors are proteins. These transcription factors form part of the transcription complex. One of these transcription factors binds directly to the TATA box in the core promoter. These transcription factors bind to the operator. These transcription factors bind to enhancer sequences that may be distant from the promoter....
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the...
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
Question 20 TFIID is a. a protein and a transcription factor. b. an enzyme. c. a...
Question 20 TFIID is a. a protein and a transcription factor. b. an enzyme. c. a promoter and a transcription factor. d. a promoter. e. part of the TATA box. Question 19 Without maintenance methyltransferase, the changes made by DNA methyltransferase would not a. be able to increase transcription of a gene. b. be passed on from one generation to the next c. coordinate regulation across different genes. d. produce microRNA. e. be able to decrease transcription of a gene....
If we placed a bacterium into a medium that contained neither lactose nor glucose (let's give...
If we placed a bacterium into a medium that contained neither lactose nor glucose (let's give them arabinose instead), what will be the level of transcription from the lac operon? O A OFF (fully repressed, but not activated) O B BASAL (neither repressed nor activated) O C HIGH (not repressed, but activated) O D OFF (both repressed and activated, but the repressor "wins") Under what conditions do we get maximum transcription from the lac operon? O A When both glucose...
Sorry for bad grammar, English is not my native language. For question 11 what is a...
Sorry for bad grammar, English is not my native language.
For question 11 what is a haploid yeast cell? If the cell lacks
the promoter in the first then will it always be true
that expression will not occur despite the UAS sequence?
What is a UAS sequence?
For question number 13 response b how/why when a promoter is
closer to a consensus sequence more transcription occurs?
11. Galactose dependent transcription is regulated by the Gal4 transcription factor which binds...
18. As glucose is catabolized, the energy is captured by: a. forming ADP and by oxidizing...
18. As glucose is catabolized, the energy is captured by: a. forming ADP and by oxidizing adenine dinucleotides b. forming ATP and by oxidizing adenine dinucleotides c. forming ADP and by reducing adenine dinucleotides d. forming ATP and by reducing adenine dinucleotides e. forming ATP and by transforming adenine dinucleotides 19. Green plantsmost of the green light to which they are exposed. In the presence of pure green light, plants can still drive photosynthesis, with the aid of the a....
Answer the questions: Question 1 Transcription begins at the..... a. operon o b. repressor c. genome...
Answer the questions:
Question 1 Transcription begins at the..... a. operon o b. repressor c. genome 17d. promoter Question 2 0.5 points Save RNA is synthesized on a DNA template in a process called replication, DNA polymerase translation, RNA polymerase transcription, RNA polymerise t ranscription, DNA polymerase Question 3 Which eukaryotic RNA polymerase makes tRNA's? a RNA polymerase IIIb. Any of these RNA polymerase I od RNA polymerase II A Moving to another question will save this response. Question 4...
GAL80 loss-of-function mutants express the GAL1, 2, 7, 10 genes constitutively in the absence of galactose) because A. transcriptional activation by GAL4 is not inhibited B. GAL80 is not present to block GAL4 DNA-binding C. GAL80 cannot respond to GAL3 signaling D. A and B E. B and C TB 68
Please explain your answers as best as you can. I want to be
able to understand.
The first drawing below represents the transcription start site and upstream region of a eukaryotic gene. In addition, four deletion constructs are shown, where the unboxed area represents a deletion of upstream DNA. Note the location of the +1 start site and the promoter, which includes the TATA box Non-deleted DNA template and DNA of the various deletions constructs were added to both systems...
Classify each statement according to whether it applies to general transcription factors only, specific transcription factors only, both, or neither These transcription factors are found in both prokaryotes and eukaryotes. These transcription factors are proteins. These transcription factors form part of the transcription complex. One of these transcription factors binds directly to the TATA box in the core promoter. These transcription factors bind to the operator. These transcription factors bind to enhancer sequences that may be distant from the promoter....
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
Question 20 TFIID is a. a protein and a transcription factor. b. an enzyme. c. a promoter and a transcription factor. d. a promoter. e. part of the TATA box. Question 19 Without maintenance methyltransferase, the changes made by DNA methyltransferase would not a. be able to increase transcription of a gene. b. be passed on from one generation to the next c. coordinate regulation across different genes. d. produce microRNA. e. be able to decrease transcription of a gene....
If we placed a bacterium into a medium that contained neither lactose nor glucose (let's give them arabinose instead), what will be the level of transcription from the lac operon? O A OFF (fully repressed, but not activated) O B BASAL (neither repressed nor activated) O C HIGH (not repressed, but activated) O D OFF (both repressed and activated, but the repressor "wins") Under what conditions do we get maximum transcription from the lac operon? O A When both glucose...
Sorry for bad grammar, English is not my native language.
For question 11 what is a haploid yeast cell? If the cell lacks
the promoter in the first then will it always be true
that expression will not occur despite the UAS sequence?
What is a UAS sequence?
For question number 13 response b how/why when a promoter is
closer to a consensus sequence more transcription occurs?
11. Galactose dependent transcription is regulated by the Gal4 transcription factor which binds...
18. As glucose is catabolized, the energy is captured by: a. forming ADP and by oxidizing adenine dinucleotides b. forming ATP and by oxidizing adenine dinucleotides c. forming ADP and by reducing adenine dinucleotides d. forming ATP and by reducing adenine dinucleotides e. forming ATP and by transforming adenine dinucleotides 19. Green plantsmost of the green light to which they are exposed. In the presence of pure green light, plants can still drive photosynthesis, with the aid of the a....
Answer the questions:
Question 1 Transcription begins at the..... a. operon o b. repressor c. genome 17d. promoter Question 2 0.5 points Save RNA is synthesized on a DNA template in a process called replication, DNA polymerase translation, RNA polymerase transcription, RNA polymerise t ranscription, DNA polymerase Question 3 Which eukaryotic RNA polymerase makes tRNA's? a RNA polymerase IIIb. Any of these RNA polymerase I od RNA polymerase II A Moving to another question will save this response. Question 4...
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