Question

Sorry for bad grammar, English is not my native language.

For question 11 what is a haploid yeast cell? If the cell lacks the promoter in the first then will it always be true that  expression will not occur despite the UAS sequence? What is a UAS sequence?

For question number 13 response b how/why when a promoter is closer to a consensus sequence more transcription occurs?

11. Galactose dependent transcription is regulated by the Gal4 transcription factor which binds to the UAS sequence in the promoter of Gal-responsive genes, such as (PGM). A haploid yeast cell lacks the PGM promoter. A plasmid is then introduced into this cell which possesses the UAS sequence. What do we expect in to see regarding PGM expression in this cell? phosphoglucomutase a. PGM expression will occur norma b. PGM expression will occur, but will no longer be galactose dependent. c. PGM expression will occur, but the protein may not be functional. d. PGM expression will occur, but will not require Gal4. PGM expression will not occur. e. 12. The enzyme Hexokinase catalyzes the first step in glycolysis. Increasing transcription of at offects in the cells? 13. What of the following is not true regarding consensus sequences? a. A consensus sequence is defined as a common sequence of nucleotides or amino acids found by comparing similar regions from different genes or genomes. b. In promoters, the closer a sequence is to the consensus sequence, the more transcription will occur at that gene. c. A consensus sequence is important for dictating where translation begins on the MRNA. d. A consensus sequence is important for dictating how histones associate with DNA. e. All of the above are true. mulated hy the renressible arg operon. Which of the

Answer 1

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