Homework Answers
Q3)
It would always be off because GAL4 activates transcription
Q4)
It would be permanently on because GAL80 inhibits transcription in the absence of Galactose.
Q5)
It would be permanently off because GAL3 is needed to bind Galactose.
The regulation of this Operon involves the activation of trancrption by GAL4 binding to UAS (Ustream Activator Sequences), while GAL80 is a repressor of transcription. However, in the presence of Galactose, GAL3 binds Galactose, and prevents GAL80 from inhibiting gene expression.
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Question 3 1 pts What would happen to the expression of this gene if this cell...
What would happen to the expression of this gene if this cell did not have any...
What would happen to the expression of this gene if this cell
did not have any GAL4 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL80 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL3 protein and why?
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down...
Describe how the expression of this gene is regulated. Include the roles of all key players...
Describe how the expression of this gene is regulated. Include
the roles of all key players (GAL3, GAL4, GAL80, Gene promoter, RNA
polymerase, and transcription factors) illustrated.
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down lactose in yeast is regulated. The top image illustrates the key regulators of the process. The bottom two panels illustrate what happens in the presence and absence of galactose. Describe how the expression...
GAL3 protein binds to GAL80 and frees up GAL4 to bind to UAS and activate transcription...
GAL3 protein binds to GAL80 and frees up GAL4 to bind to UAS and activate transcription of genes involved in galactose metabolism (see text). Assuming that you did not have prior knowledge of GAL3 protein but knew about GAL4 and GAL80, what experiments (genetic screens or selection experiments) would you perform to identify the GAL3 gene and additional genes involved in the regulation of GAL4?
Examine the figure below. What would be the effect on transcription if a mutation occurred in...
Examine the figure below. What would be the effect on
transcription if a mutation occurred in the gene that encodes GAL3,
so that no functional GAL3 was produced?
GAL4 UASG GAL80 In the absence of galactose, GAL80 blocks GAL4 from activating transcription. TATA Basal transcription apparatus Galactose GAL3 GAL80 GAL3 Galactose When galactose is present, it binds to GAL3 and brings about a change in the conformation of GAL80 GAL80 no longer binds to GAL4. GAL4 TATA 3 GAL4 can...
4. Examine the figure below. What would be the effect on transcription if a mutation occurred...
4. Examine the figure below. What would be the effect on transcription if a mutation occurred in the gene that encodes GAL3, so that no functional GAL was produced? GAL4 UASC GAL80 In the absence of galactose, GAL80 blocks GAL4 from activating transcription TATA Basal transcription apparatus Galactose GAL3 GAL80 GAL3 2 When galactose is present, it binds to GAL3 and brings about a change in the Galactose conformation of GAL80. GAL80 no longer binds to GAL4. GAL4 TATA 3...
1. (2pt) Choose the correct statement with respect to transcription of the GAL10 gene in this...
1. (2pt) Choose the correct statement with respect to transcription of the GAL10 gene in this yeast strain in the absence of galactose: GAL 10 WILL be transcribed because Gal4 is bound to Gal3 GAL 10 WILL be transcribed because Gal80 is bound to Gal4 GAL 10 WILL be transcribed because Gal80 is bound to Gal3 - GAL10 will NOT be transcribed because Gal80 is bound to Gal4 - GAL10 will NOT be transcribed because Gal3 is bound to Gal4...
Answer all questions and explain in detail. 1) GAL80 loss-of-function mutants express GAL1, 2, 7, 10,...
Answer all questions and explain in detail. 1) GAL80 loss-of-function mutants express GAL1, 2, 7, 10, genes constitutively (in the absence of galactose) because a transcriptional activation by GAL4 is not inhibited b GAL80 is not present to block GAL4 DNA-binding c GAL80 cannot respond to GAL3 signaling d A and B e B and C 2) Gal80-; GAL4- double mutants express the GAL1, 7, 10 genes a only In the presence of galactose b only in the absence of...
Sorry for bad grammar, English is not my native language. For question 11 what is a...
Sorry for bad grammar, English is not my native language.
For question 11 what is a haploid yeast cell? If the cell lacks
the promoter in the first then will it always be true
that expression will not occur despite the UAS sequence?
What is a UAS sequence?
For question number 13 response b how/why when a promoter is
closer to a consensus sequence more transcription occurs?
11. Galactose dependent transcription is regulated by the Gal4 transcription factor which binds...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dyst...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
1. What would happen to a gene if the stop codon (UAA, UAG, or UGA) were...
1. What would happen to a gene if the stop codon (UAA, UAG, or UGA) were mitated so that the first position in the codon became a “C” (CAA, CAG, CGA)? a) the protein product of the gene would be similar than normal b) either the immature RNA transcript or the mRNA will be degraded by nonsense mediated RNA decay c) its mature messenger RNA would be lengthened d) its immature RNA would be lengthened 2. Without a promoter region,...
What would happen to the expression of this gene if this cell
did not have any GAL4 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL80 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL3 protein and why?
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down...
Describe how the expression of this gene is regulated. Include
the roles of all key players (GAL3, GAL4, GAL80, Gene promoter, RNA
polymerase, and transcription factors) illustrated.
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down lactose in yeast is regulated. The top image illustrates the key regulators of the process. The bottom two panels illustrate what happens in the presence and absence of galactose. Describe how the expression...
Examine the figure below. What would be the effect on
transcription if a mutation occurred in the gene that encodes GAL3,
so that no functional GAL3 was produced?
GAL4 UASG GAL80 In the absence of galactose, GAL80 blocks GAL4 from activating transcription. TATA Basal transcription apparatus Galactose GAL3 GAL80 GAL3 Galactose When galactose is present, it binds to GAL3 and brings about a change in the conformation of GAL80 GAL80 no longer binds to GAL4. GAL4 TATA 3 GAL4 can...
4. Examine the figure below. What would be the effect on transcription if a mutation occurred in the gene that encodes GAL3, so that no functional GAL was produced? GAL4 UASC GAL80 In the absence of galactose, GAL80 blocks GAL4 from activating transcription TATA Basal transcription apparatus Galactose GAL3 GAL80 GAL3 2 When galactose is present, it binds to GAL3 and brings about a change in the Galactose conformation of GAL80. GAL80 no longer binds to GAL4. GAL4 TATA 3...
1. (2pt) Choose the correct statement with respect to transcription of the GAL10 gene in this yeast strain in the absence of galactose: GAL 10 WILL be transcribed because Gal4 is bound to Gal3 GAL 10 WILL be transcribed because Gal80 is bound to Gal4 GAL 10 WILL be transcribed because Gal80 is bound to Gal3 - GAL10 will NOT be transcribed because Gal80 is bound to Gal4 - GAL10 will NOT be transcribed because Gal3 is bound to Gal4...
Sorry for bad grammar, English is not my native language.
For question 11 what is a haploid yeast cell? If the cell lacks
the promoter in the first then will it always be true
that expression will not occur despite the UAS sequence?
What is a UAS sequence?
For question number 13 response b how/why when a promoter is
closer to a consensus sequence more transcription occurs?
11. Galactose dependent transcription is regulated by the Gal4 transcription factor which binds...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
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