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Which one is NOT true of RNA seq? a. It requires direct sequencing of nucleic acids....

Which one is NOT true of RNA seq?

a. It requires direct sequencing of nucleic acids.

b. It can identify the expression of large numbers of genes at the same time.

c. It is based on annealing between RNA and the nucleotide oligos punched on a glass slide in the flow-cell.

d. It can determine the changes in gene expression during development.

The amphibian egg is radially symmetric along the animal-vegetal axis. What ‘breaks’ this symmetry?

a. Localization of maternal RNAs to the animal pole

c. Cleavage

b. Fertilization

d. Localization of maternal RNAs to the vegetal pole

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Answer #1

RNA-Seq (RNA sequencing), also called whole transcriptome shotgunsequencing (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time. RNA-Seq is used to analyze the continually changing cellular transcriptome.

B

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Answer #2
It is less accurate than microarrays.
source: Principles of Development 4th or 5th edition by Lewis Wolpert et al. Oxford University Press.
answered by: Lilly James
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