20. In most proteins this amino acid's side chain is found clustered away from the aqueous exterior of the protein as a result of which interaction?
a) Hydrophobic effect
b) Charge-charge (ionic)
c) Dipole-dipole
d) Van der Waals (dispersion)
|
A protein has a pI of 6.5. If I want to use an anion exchange resin (there is a +ve charge on the resin) to bind the protein, what pH could I perform the separation at? |
a) pH 8
b) pH 6.5
c) pH 5
d) The pH doesn't matter
|
A protein has a pI of 7, what will be the net charge on the protein at pH 5? |
a) negative
b) positive
c) no net charge
d) both positive and negative
Homework Answers
20. Answer:- a
The organic groups which are water repellent are called hydrophobic groups. These try to remain away from water.
Examples of such groups include:- hydrocarbon
On the other hand the groups which are water attractive are called hydrophilic groups.
Example:- -NH2 group
20. Answer:- a
The organic groups which are water repellent are called hydrophobic groups. These try to remain away from water.
Examples of such groups include:- hydrocarbon
On the other hand the groups which are water attractive are called hydrophilic groups.
Example:- -NH2 group
Add Answer to:
20. In most proteins this amino acid's side chain is found
clustered away from the aqueous...
1. Which of the following amino acids has the most hydrophobic side chain? a). E b)....
1. Which of the following amino acids has the most hydrophobic side chain? a). E b). F c). S d). W e). Y 2. Which type of non covalent interaction would you expect to be present between Cl- and H2O? a). Ionic interactions ("salt links") b). Hydrogen bonds c). Van der Waals interactions d). Ion-dipole interactions For the first question, I have F and W both being hydrophobic chains but am unsure why or how to tell which one would...
The pK of the side chain of amino acid X in a polypeptide is normally in...
The pK of the side chain of amino acid X in a polypeptide is normally in the range of 9-10 and carries a positive charge when protonated. Suppose you have a soluble globular protein and you find there is an X that has a pK of 6.5. What is the most likely reason for such a large drop in pK? Circle the correct answer. a) X is on the surface of the protein where it ion pairs with the carboxylate...
2. (4 points) Multiple choice. Circle the best answer. In anion exchange chromatography, the charge on...
2. (4 points) Multiple choice. Circle the best answer. In anion exchange chromatography, the charge on the column is We would expect a protein with ___ to bind most tightly to this column. a) positive, pH < PI b) positive , pH = pi c) positive , pH > pl d) negative , pH < PI e) negative , pH = PI f) negative , pH > PI
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a pr...
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a protein? -CH-CH3 он CH2-CH-CH3 он CH3 11) What process occurs when heat, acids, bases, and heavy metal ions cause loss of biological function of a protein? 12) Answer the following questions regarding enzyme activity: a) The optimum temperature and pH for most biological enzymes is b) The molecules to which an enzyme binds are called the c) The...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
26. Which of the following classification does not match the amino acid side chain A) Contains an basic group/ lysi...
26. Which of the following classification does not match the amino acid side chain A) Contains an basic group/ lysine B) It is polar C) Forms disulfide bond/ cysteine D) Forms hydrogen bonds with neighbors/ alanine serine 27. All amino acids found in proteins are L-amino acids EXCEPT the achiral. A) glutamate B) Lysine C) glyeine D) Alamine 28. The plH at which the positive and negative charges of an amino acid balance each ofher is called the A) isotonic...
Page < 2 > of 5 0 8) In the most stable conformation of tert-butylcyclohexane, how...
Page < 2 > of 5 0 8) In the most stable conformation of tert-butylcyclohexane, how many axial hydrogen atoms are present? a. O Eb. 2 e. 6 9) Which statement is true concerning the mechanism for reaction between HBr and H2C=CH2 to form bromoethane? a the reaction forms mechanism shows electron movement using full arrows, e.g. b. the reaction involves a slow, rate-determining formation of a radical intermediate c. the reaction involves homolysis d. In the first step, HBr...
11 Department of Biological Sciences BIOCHEMISTRY Test 2.2018 H. Nucleic Acid, Sequencing of DNA: Amino Acids...
11 Department of Biological Sciences BIOCHEMISTRY Test 2.2018 H. Nucleic Acid, Sequencing of DNA: Amino Acids Hydrolysis of salt Laboratory buffers, Polyprotie acids QUESTIONS 1. The DNA strand complementary to the strand 3-GTAGCGTAT-Y' would have the sequence a SLTACOCATAT-3 b.3.CATOXICATA-S c. 3-ATGCGTATA-S" ATATGCGTAS 2. When cytosine is treated with bisulfite, the amino group is replaced with a carbonyl group. Identify the resulting base a. adenine b. guanine c uracil d. thyminee. typoxanthine 3. How many amino acids would be in...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has its own characteristic atom in which a. the atomic mass is constant. b. the atomic number is constant. c. the mass number is constant. d. Two of the above are correct. e. All of the above are correct. 2. Which of the following is not a trace element in the human body? a. iodine b. zinc c. iron d. calcium e. fluorine 3. A...
What are the instruments that have been utilized for the review article discussions? ` 1. Introduction...
What are the instruments that have been utilized for the
review article discussions?
`
1. Introduction In recent years, nanoclays have been the object of particular interest for many scientists and researchers in chemistry, physics, engineering and biology due to their excellent properties as well as their sustain- ability [1-3]. For instance, they represent the starting point to the de velopment of smart materials for drug delivery (4-9), food packaging [10-12), environmental remediation and wastewater treatment [13], cultural heritage [14–17and...
2. (4 points) Multiple choice. Circle the best answer. In anion exchange chromatography, the charge on the column is We would expect a protein with ___ to bind most tightly to this column. a) positive, pH < PI b) positive , pH = pi c) positive , pH > pl d) negative , pH < PI e) negative , pH = PI f) negative , pH > PI
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a protein? -CH-CH3 он CH2-CH-CH3 он CH3 11) What process occurs when heat, acids, bases, and heavy metal ions cause loss of biological function of a protein? 12) Answer the following questions regarding enzyme activity: a) The optimum temperature and pH for most biological enzymes is b) The molecules to which an enzyme binds are called the c) The...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
26. Which of the following classification does not match the amino acid side chain A) Contains an basic group/ lysine B) It is polar C) Forms disulfide bond/ cysteine D) Forms hydrogen bonds with neighbors/ alanine serine 27. All amino acids found in proteins are L-amino acids EXCEPT the achiral. A) glutamate B) Lysine C) glyeine D) Alamine 28. The plH at which the positive and negative charges of an amino acid balance each ofher is called the A) isotonic...
Page < 2 > of 5 0 8) In the most stable conformation of tert-butylcyclohexane, how many axial hydrogen atoms are present? a. O Eb. 2 e. 6 9) Which statement is true concerning the mechanism for reaction between HBr and H2C=CH2 to form bromoethane? a the reaction forms mechanism shows electron movement using full arrows, e.g. b. the reaction involves a slow, rate-determining formation of a radical intermediate c. the reaction involves homolysis d. In the first step, HBr...
11 Department of Biological Sciences BIOCHEMISTRY Test 2.2018 H. Nucleic Acid, Sequencing of DNA: Amino Acids Hydrolysis of salt Laboratory buffers, Polyprotie acids QUESTIONS 1. The DNA strand complementary to the strand 3-GTAGCGTAT-Y' would have the sequence a SLTACOCATAT-3 b.3.CATOXICATA-S c. 3-ATGCGTATA-S" ATATGCGTAS 2. When cytosine is treated with bisulfite, the amino group is replaced with a carbonyl group. Identify the resulting base a. adenine b. guanine c uracil d. thyminee. typoxanthine 3. How many amino acids would be in...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has its own characteristic atom in which a. the atomic mass is constant. b. the atomic number is constant. c. the mass number is constant. d. Two of the above are correct. e. All of the above are correct. 2. Which of the following is not a trace element in the human body? a. iodine b. zinc c. iron d. calcium e. fluorine 3. A...
What are the instruments that have been utilized for the
review article discussions?
`
1. Introduction In recent years, nanoclays have been the object of particular interest for many scientists and researchers in chemistry, physics, engineering and biology due to their excellent properties as well as their sustain- ability [1-3]. For instance, they represent the starting point to the de velopment of smart materials for drug delivery (4-9), food packaging [10-12), environmental remediation and wastewater treatment [13], cultural heritage [14–17and...
Most questions answered within 3 hours.
-
1. Nimbus, Inc. produces and sells brooms. This table shows the
relationship between the number of...
asked 31 seconds ago -
A gas occupies 200. mL in a piston. If the pressure of the
piston were decreased...
asked 17 minutes ago -
A fossil is found to have a 14C level of 71.0% compared to
living organisms. How...
asked 20 minutes ago -
Many communist or socialist countries have a department that
addresses public health as well as the...
asked 22 minutes ago -
the following questions are either true or false answers
1. The Central Limit Theorem allows one...
asked 23 minutes ago -
The patient recovery time from a particular surgical procedure
is normally distributed with a mean of...
asked 29 minutes ago -
Human relations refer to the way a company arranges people,
jobs, and communications so that work...
asked 46 minutes ago -
Python Program: Design the logic for and implement a program
that merges the two files into...
asked 44 minutes ago -
The specific radiocarbon activity of a sample of wood is 6.25
gms dpm/gm of carbon. The...
asked 49 minutes ago -
An aqueous magnesium chloride solution is made by dissolving
6.96 moles of MgCl2 in sufficient water...
asked 52 minutes ago -
Ken believes the average age of men who come to get a haircut at
his barber...
asked 1 hour ago -
(Ratio Analysis): Last year Co. XYZ had sales of $ 400,000, with
“cost of goods sold”...
asked 1 hour ago