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2- You have two components, A and B , if Da = 500 and DB = 480, then, which one come out of the column first?
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Answer #1

A will come out of the column first then component B will come out.

Smaller D means the corresponding smaller component will spend more time in the spaces present in the column. So it will take a longer time to elute out. While a larger component is more likely to avoid such small spaces in a column.

This implies B with DB = 480 will spend comparably more time in column compare to the component A with DA = 500.

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