In a chromatographic separation of two components A and B. Component A has a value of...
Homework Answers
Rf value is the ratio of distance travelled by the solute to distance travelled by the solvent.
Compound A has greater Rf value which indicates that compound A has travelled larger distance compared to compound b.
This clearly indicates that the compound B has higher affinity than A for mobile phase and compound B has higher affinity towards stationary phase than A.
Option B is the right answer.
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In
a chromatographic separation of two components A and B. Component A
has a value of...
4. Consider a mixture of two components X and Y that you want to analyze by...
4. Consider a mixture of two components X and Y that you want to analyze by paper chromatography. If the chemical affinity of X for the stationary phase is greater than that of Y, and the chemical affinity of X for the mobile phase is less than that for Y, which substance will have the largest Rf value upon analysis of the developed chromatogram. Explain your answer.
Question 28 1 points Given below is a diagram of separation of two components of a...
Question 28 1 points Given below is a diagram of separation of two components of a mixture in a chromatographic column. From the diagram inter which component has lesser afinity to the stationary pha Mobile phase Component w Component Component Component Component X Component z Component Y 0 0 NT & E 0 0 0 O All the above Question 29 Select the incorrect statement from the following options The spots of colorless compounds are invisible to the eyes The...
Question 1: (column chromatography) a. What effect on the chromatographic separation is expected ...
Question 1: (column chromatography) a. What effect on the chromatographic separation is expected if solvent level is below the adsorbent? b. Once the chromatographic column was prepared, why was it important to allow the level of the liquid in the column to drop to the level of the stationary phase before applying the solution of the compounds to be separated? c. Name two methods of carrying out the column chromatography technique? d. What are the two methods of packing a...
What effect will the following factors have on a column chromatographic separation with alumina as the...
What effect will the following factors have on a column chromatographic separation with alumina as the stationary phase? A) methanol is used as the initial eluting solvent B) The level of the eluting solvent falls below the top of the column? C) Heptane is used as the initial eluting solvent
help needed with this problem set Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (...
help needed with this problem set
Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (top) are described in the figure title and the insert. You only need the lecture notes and/or your personal experience to answer those questions. Mobile phase: 30 % MeCN, 70 % TFA, 0.1 M (pH 2.0) low rate: 1.0 mL/min 2Benzok Acid Maleic Aci 1. (a) Which component has the...
Please explain the concept of chromatographic separation and formulas in the above question. 2) If a...
Please explain the concept of chromatographic separation and
formulas in the above question.
2) If a big centrifuge with the bowl diameter of 1m rotates at
60 rpm, at what speed (rpm) does a smaller centrifuge with a
diameter of 0.5 m need to be operated for achieving the same
separation factor?
(a) 85
(b) 120
(c) 30
(d) 42
3) What is the linear flow rate (superficial velocity in cm/h)
in a chromatographic column of 1.0 cm inner diameter...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
1. Select an appropriate type of column for separating these steroids and give the rationale for...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
An electrical device has two components, A and B. Each component can be "good" or can...
An electrical device has two components, A and B. Each component can be "good" or can be in a "shorted" or "open-mode" failure state. Let's assume that component A has probability .6 or being good and probability .2 of being in each of the failure states, and that component B has probability .7 of being good, .2 of "open" failure, and .1 for "shorted" failure. Furthermore, we assume the two components function independently. Current can pass through a device if...
PRELABORATORY ASSIGNMENT 1. Provide the key term that corresponds to each of the following definitions. (a)...
PRELABORATORY ASSIGNMENT 1. Provide the key term that corresponds to each of the following definitions. (a) the study of compounds derived from plants and animals (b) a method for separating a mixture into its components as a result of a varying attraction of compounds for a mobile solvent on a stationary solid (c) the leading edge of the solvent, which travels from the bottom of the developing chamber to the upper portion of the chromatogram (d) refers to solvent that...
Question 28 1 points Given below is a diagram of separation of two components of a mixture in a chromatographic column. From the diagram inter which component has lesser afinity to the stationary pha Mobile phase Component w Component Component Component Component X Component z Component Y 0 0 NT & E 0 0 0 O All the above Question 29 Select the incorrect statement from the following options The spots of colorless compounds are invisible to the eyes The...
help needed with this problem set
Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (top) are described in the figure title and the insert. You only need the lecture notes and/or your personal experience to answer those questions. Mobile phase: 30 % MeCN, 70 % TFA, 0.1 M (pH 2.0) low rate: 1.0 mL/min 2Benzok Acid Maleic Aci 1. (a) Which component has the...
Please explain the concept of chromatographic separation and
formulas in the above question.
2) If a big centrifuge with the bowl diameter of 1m rotates at
60 rpm, at what speed (rpm) does a smaller centrifuge with a
diameter of 0.5 m need to be operated for achieving the same
separation factor?
(a) 85
(b) 120
(c) 30
(d) 42
3) What is the linear flow rate (superficial velocity in cm/h)
in a chromatographic column of 1.0 cm inner diameter...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
PRELABORATORY ASSIGNMENT 1. Provide the key term that corresponds to each of the following definitions. (a) the study of compounds derived from plants and animals (b) a method for separating a mixture into its components as a result of a varying attraction of compounds for a mobile solvent on a stationary solid (c) the leading edge of the solvent, which travels from the bottom of the developing chamber to the upper portion of the chromatogram (d) refers to solvent that...
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