Question

Enzyme Reactions and Kinetics Q6.3: What kinetic parameter reveals the most information about how well an enzyme works? Describe this kinetic parameter in terms of the turnover number and the Michaelis constant and provide an explanation for why triosephosphate isomerase is considered to be 100,000 better than pepsin when this kinetic parameter is used to compare these two enzymes.

Answer 1

From Micheal menton enzyme kinetics we have

Where 

K_cat is turn over number

[E]_T is total enzyme concnetration

The parameter that is used to describe how well a catalyst works is known as catalytic efficiency =K_cat/K_m

_{Triphosphate isomerase is considered way better than pepsin because Km value i.e concentration of substrate required to reach half of maximumvalue is very less for triphostphate and very low quanitty of it catalysis the reaction to significantly higher extent making its catalytic efficiceny very very high than pepsin.}