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Identify and describe the role of two components in the transformation of E. coli in this...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of lac operon in E.coli. b. Role of CAP and cAMP the activator binding site c. Role of Lac Repressor and Operator and Inducer d. Role of Looping e. Effect of mutations f. Recessive v dominant mutations g. Complementing v noncomplementing mutations. Describe the regulation of the trp operon in E. coli. Describe at least three kinds of molecular switches involved in gene regulation. What...
Describe the role of the proA gene in E. coli (i.e., what does the resulting protein do? Cite primary article pls
Describe the role of the proA gene in E. coli (i.e., what does the resulting protein do? Cite primary article pls, no use without citations
During the course of an E. coli transformation experiment, a student forgot to identify the tube for that received the kanamycin-resistant plasmid. The student decided to incubate the plates, thinking that he/she will be able to determine from the results which culture tube contained cells that may have undergone transformation. Which plate would be most likely to indicate transformed cells? a. A plate with a lawn of cells growing on LB agar with kanamycin b. A plate with 100 colonies...
4. You have prepared a batch of competent E coli cells and found its transformation efficiency is 106 per μg plasmid DNA. A professional scientists prepared competent E. coli cells having a transformation efficiency of about 108 cells/ g plasmid DNA Calculate % score of your E. coli transformation efficiency (Τ.Ε.).
Describe ligation reaction. Discuss the possible products of ligation reaction. What happened to e coli that did not take any the vector? Drug resistance? What is the maximum transformation efficiency that can be expected? What things can influence the transformation efficiency and ligation reaction using 2 endonuclease for subcloning instead of one?
Albert is performing bacterial transformation. He has E. coli cells in 495 μl of CaCl2 transformation solution, and he adds 5 μl of 10 μg/ml plasmid. What is the final concentration of plasmid in his transformation reaction? Express your answer in μg/ml. Round your answer to the first decimal place.
BUSN 630: Week 5 Explain the concept of e-collaborations and virtual organizations Identify and describe resource sharing and distribution strategies Identify and discuss the features of successful virtual organizations Identify and describe the role of virtualization in organizational change and transformation Prompt: Online banking and financial institutions are two examples of hybrid virtual organizations that are heavily dependent on technology to support B2C and B2B relationships. Select an organization (this is different than the organization you selected for the research...
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10 points) 1) RNase H 2) Gyrase 3) Primase 4) Dna C
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during protein synthesis is: a. aid in initiation of protein synthesis b. contributes to the process of translocation during the elongation stage of protein synthesis c. helps to form the A site within the ribosome d. actively forms a peptide bond e. none of the above P) Spontaneous mutations can be caused by: a. incorrect translation b. telomerase c. chemical mutagens d. tautomeric shifts e....